Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/354912
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampMofatto, Luciana Souto-
dc.contributor.authorunicampVidal, Ramon Oliveira-
dc.contributor.authorunicampCarazzolle, Marcelo Falsarella-
dc.typeArtigopt_BR
dc.titleIdentification of candidate genes for drought tolerance in coffee by high-throughput sequencing in the shoot apex of different Coffea arabica cultivarspt_BR
dc.contributor.authorMofatto, Luciana Souto-
dc.contributor.authorCarneiro, Fernanda de Araújo-
dc.contributor.authorVieira, Natalia Gomes-
dc.contributor.authorDuarte, Karoline Estefani-
dc.contributor.authorVidal, Ramon Oliveira-
dc.contributor.authorAlekcevetch, Jean Carlos-
dc.contributor.authorCotta, Michelle Guitton-
dc.contributor.authorVerdeil, Jean-Luc-
dc.contributor.authorLapeyre-Montes, Fabienne-
dc.contributor.authorLartaud, Marc-
dc.contributor.authorLeroy, Thierry-
dc.contributor.authorBellis, Fabien De-
dc.contributor.authorPot, David-
dc.contributor.authorRodrigues, Gustavo Costa-
dc.contributor.authorCarazzolle, Marcelo Falsarella-
dc.contributor.authorPereira, Gonçalo Amarante Guimarães-
dc.contributor.authorAndrade, Alan Carvalho-
dc.contributor.authorMarraccini, Pierre-
dc.subjectCoffea arabicapt_BR
dc.subjectEstudos de associação genéticapt_BR
dc.subject.otherlanguageCoffea arabicapt_BR
dc.subject.otherlanguageGenetic association studiespt_BR
dc.description.abstractDrought is a widespread limiting factor in coffee plants. It affects plant development, fruit production, bean development and consequently beverage quality. Genetic diversity for drought tolerance exists within the coffee genus. However, the molecular mechanisms underlying the adaptation of coffee plants to drought are largely unknown. In this study, we compared the molecular responses to drought in two commercial cultivars (IAPAR59, drought-tolerant and Rubi, drought-susceptible) of Coffea arabica grown in the field under control (irrigation) and drought conditions using the pyrosequencing of RNA extracted from shoot apices and analysing the expression of 38 candidate genes. Pyrosequencing from shoot apices generated a total of 34.7 Mbp and 535,544 reads enabling the identification of 43,087 clusters (41,512 contigs and 1,575 singletons). These data included 17,719 clusters (16,238 contigs and 1,575 singletons) exclusively from 454 sequencing reads, along with 25,368 hybrid clusters assembled with 454 sequences. The comparison of DNA libraries identified new candidate genes (n = 20) presenting differential expression between IAPAR59 and Rubi and/or drought conditions. Their expression was monitored in plagiotropic buds, together with those of other (n = 18) candidates genes. Under drought conditions, up-regulated expression was observed in IAPAR59 but not in Rubi for CaSTK1 (protein kinase), CaSAMT1 (SAM-dependent methyltransferase), CaSLP1 (plant development) and CaMAS1 (ABA biosynthesis). Interestingly, the expression of lipid-transfer protein (nsLTP) genes was also highly up-regulated under drought conditions in IAPAR59. This may have been related to the thicker cuticle observed on the abaxial leaf surface in IAPAR59 compared to Rubi. The full transcriptome assembly of C. arabica, followed by functional annotation, enabled us to identify differentially expressed genes related to drought conditions. Using these data, candidate genes were selected and their differential expression profiles were confirmed by qPCR experiments in plagiotropic buds of IAPAR59 and Rubi under drought conditions. As regards the genes up-regulated under drought conditions, specifically in the drought-tolerant IAPAR59, several corresponded to orphan genes but also to genes coding proteins involved in signal transduction pathways, as well as ABA and lipid metabolism, for example. The identification of these genes should help advance our understanding of the genetic determinism of drought tolerance in coffee.pt_BR
dc.relation.ispartofBMC plant biologypt_BR
dc.publisher.cityLondonpt_BR
dc.publisher.countryReino Unidopt_BR
dc.publisherSpringer Naturept_BR
dc.date.issued2016-
dc.date.monthofcirculationApr.pt_BR
dc.language.isoengpt_BR
dc.description.volume16pt_BR
dc.description.issuenumber94pt_BR
dc.rightsAbertopt_BR
dc.sourceWOSpt_BR
dc.identifier.eissn1471-2229pt_BR
dc.identifier.doi10.1186/s12870-016-0777-5pt_BR
dc.identifier.urlhttps://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-016-0777-5pt_BR
dc.description.sponsorshipCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE MINAS GERAIS - FAPEMIGpt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DEpt_BR
dc.description.sponsordocumentnumberSem informaçãopt_BR
dc.description.sponsordocumentnumberSem informaçãopt_BR
dc.description.sponsordocumentnumberSem informaçãopt_BR
dc.description.sponsordocumentnumber2013/08293-7pt_BR
dc.date.available2021-01-28T20:01:18Z-
dc.date.accessioned2021-01-28T20:01:18Z-
dc.description.provenanceSubmitted by Bruna Maria Campos da Cunha (bcampos@unicamp.br) on 2021-01-28T20:01:18Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-02-19T18:02:09Z : No. of bitstreams: 1 000374285200002.pdf: 3908761 bytes, checksum: 393f32106162eb2a28fd3972c879cd66 (MD5)en
dc.description.provenanceMade available in DSpace on 2021-01-28T20:01:18Z (GMT). No. of bitstreams: 0 Previous issue date: 2016en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/354912-
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentDepartamento de Genética, Evolução, Microbiologia e Imunologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.subject.keywordCandidate genept_BR
dc.subject.keywordDroughtpt_BR
dc.subject.keywordDifferential gene expressionpt_BR
dc.subject.keywordRNA-Seqpt_BR
dc.subject.keywordReal-timept_BR
dc.subject.keywordPCR (RT-qPCR)pt_BR
dc.identifier.source000374285200002pt_BR
dc.creator.orcid0000-0002-2087-7251pt_BR
dc.creator.orcidSem informaçãopt_BR
dc.creator.orcid0000-0002-5474-2830pt_BR
dc.type.formArtigo de pesquisapt_BR
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