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Type: Artigo
Title: PBMCs express a transcriptome signature predictor of oxygen uptake responsiveness to endurance exercise training in men
Author: Dias, Rodrigo Goncalves
Moreira Silva, Michelle Sabrina
Duarte, Nubia Esteban
Bolani, Wladimir
Alves, Cleber Rene
Lemos Junior, Jose Ribeiro
da Silva, Jeferson Luis
de Oliveira, Patricia Alves
Alves, Guilherme Barreto
de Oliveira, Edilamar Menezes
Rocha, Cristiane Souza
Carneiro Marsiglia, Julia Daher
Negrao, Carlos Eduardo
Krieger, Eduardo Moacyr
Krieger, Jose Eduardo
Pereira, Alexandre Costa
Abstract: Peripheral blood cells are an accessible environment in which to visualize exercise-induced alterations in global gene expression patterns. We aimed to identify a peripheral blood mononuclear cell (PBMC) signature represented by alterations in gene expression, in response to a standardized endurance exercise training protocol. In addition, we searched for molecular classifiers of the variability in oxygen uptake ((V) over dotO(2)). Healthy untrained policemen recruits (n = 13, 25 +/- 3 yr) were selected. Peak (V) over dotO(2) (measured by cardiopulmonary exercise testing) and total RNA from PBMCs were obtained before and after 18 wk of running endurance training (3 times/wk, 60 min). Total RNA was used for whole genome expression analysis using Affymetrix GeneChip Human Gene 1.0 ST. Data were normalized by the robust multiarray average algorithm. Principal component analysis was used to perform correlations between baseline gene expression and (V) over dotO(2peak). A set of 211 transcripts was differentially expressed (ANOVA, P < 0.05 and fold change > 1.3). Functional enrichment analysis revealed that transcripts were mainly related to immune function, cell cycle processes, development, and growth. Baseline expression of 98 and 53 transcripts was associated with the absolute and relative (V) over dotO(2)peak response, respectively, with a strong correlation (r > 0.75, P < 0.01), and this panel was able to classify the 13 individuals according to their potential to improve oxygen uptake. A subset of 10 transcripts represented these signatures to a similar extent. PBMCs reveal a transcriptional signature responsive to endurance training. Additionally, a baseline transcriptional signature was associated with changes in (V) over dotO(2peak). Results might illustrate the possibility of obtaining molecular classifiers of endurance capacity changes through a minimally invasive blood sampling procedure
Subject: Expressão gênica
Teste de esforço
Country: Estados Unidos
Editor: American Physiological Society
Rights: Fechado
Identifier DOI: 10.1152/physiolgenomics.00072.2014
Date Issue: 2015
Appears in Collections:FCM - Artigos e Outros Documentos

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