Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/354824
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampTibo, Luiz Henrique Soares-
dc.contributor.authorunicampBertól, Jéssica Wildgrube-
dc.contributor.authorunicampBernedo-Navarro, Robert Alvin-
dc.contributor.authorunicampYano, Tomomasa-
dc.typeOutro documentopt_BR
dc.titleCytotoxic factor secreted by Escherichia coli associated with sepsis facilitates transcytosis through human umbilical vein endothelial cell monolayerspt_BR
dc.contributor.authorTibo, Luiz Henrique Soares-
dc.contributor.authorBertol, Jéssica Wildgrube-
dc.contributor.authorBernedo-Navarro, Robert Alvin-
dc.contributor.authorYano, Tomomasa-
dc.subjectTranscitosept_BR
dc.subjectCitotoxinaspt_BR
dc.subjectSepsept_BR
dc.subject.otherlanguageCytotoxinspt_BR
dc.subject.otherlanguageSepsispt_BR
dc.subject.otherlanguageTranscytosispt_BR
dc.description.abstractCulture supernatant of sepsis-associated Escherichia coli (SEPEC) isolated from patients with sepsis caused loss of intercellular junctions and elongation of human umbilical vein endothelial cells (HUVEC). The cytotoxic factor was purified from culture supernatant of SEPEC 15 (serogroup O153) by liquid chromatography process. PAGE (polyacrylamide gel electrophoresis) showed that the purified SEPEC cytotoxic factor had a molecular mass of ∼150 kDa and consisted of at least two subunits. At the concentration of 1 CD50 (40 μg/mL) did facilitate transcytosis through the HUVEC cells monolayer of SEPEC 15 as much as E. coli K12 within 30 min without affecting cell viability. These results suggest that this cytotoxic factor, named as SPF (SEPEC's permeabilizing factor), may be an important SEPEC virulence factor that facilitates bacterial access to the bloodstream.pt_BR
dc.relation.ispartofThe brazilian journal of infectious diseasespt_BR
dc.relation.ispartofabbreviationBraz. j. infect. dis.pt_BR
dc.publisher.cityBristolpt_BR
dc.publisher.countryReino Unidopt_BR
dc.publisherElsevierpt_BR
dc.date.issued2016-
dc.date.monthofcirculationMay/Junept_BR
dc.language.isoengpt_BR
dc.description.volume20pt_BR
dc.description.issuenumber3pt_BR
dc.description.firstpage298pt_BR
dc.description.lastpage302pt_BR
dc.rightsAbertopt_BR
dc.sourceSciELOpt_BR
dc.identifier.issn1413-8670pt_BR
dc.identifier.eissn1678-4391pt_BR
dc.identifier.doi10.1016/j.bjid.2016.01.003pt_BR
dc.identifier.urlhttps://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000300014pt_BR
dc.description.sponsorshipCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESpt_BR
dc.description.sponsordocumentnumberSem informaçãopt_BR
dc.date.available2021-01-27T17:01:33Z-
dc.date.accessioned2021-01-27T17:01:33Z-
dc.description.provenanceSubmitted by Bruna Maria Campos da Cunha (bcampos@unicamp.br) on 2021-01-27T17:01:33Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-02-19T18:02:06Z : No. of bitstreams: 1 S1413-86702016000300014.pdf: 1208197 bytes, checksum: 41da247ea68b0334ee29e27c15e7e8ea (MD5)en
dc.description.provenanceMade available in DSpace on 2021-01-27T17:01:33Z (GMT). No. of bitstreams: 0 Previous issue date: 2016en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/354824-
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentDepartamento de Genética, Evolução e Bioagentespt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.subject.keywordResistência elétrica transendotelialpt_BR
dc.identifier.sourceS1413-86702016000300014pt_BR
dc.creator.orcid0000-0002-3783-9331pt_BR
dc.creator.orcid0000-0002-0898-1822pt_BR
dc.creator.orcid0000-0002-8105-0418pt_BR
dc.creator.orcid0000-0002-1213-6453pt_BR
dc.type.formComunicaçãopt_BR
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