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http://repositorio.unicamp.br/jspui/handle/REPOSIP/354824
Type: | Outro documento |
Title: | Cytotoxic factor secreted by Escherichia coli associated with sepsis facilitates transcytosis through human umbilical vein endothelial cell monolayers |
Author: | Tibo, Luiz Henrique Soares Bertol, Jéssica Wildgrube Bernedo-Navarro, Robert Alvin Yano, Tomomasa |
Abstract: | Culture supernatant of sepsis-associated Escherichia coli (SEPEC) isolated from patients with sepsis caused loss of intercellular junctions and elongation of human umbilical vein endothelial cells (HUVEC). The cytotoxic factor was purified from culture supernatant of SEPEC 15 (serogroup O153) by liquid chromatography process. PAGE (polyacrylamide gel electrophoresis) showed that the purified SEPEC cytotoxic factor had a molecular mass of ∼150 kDa and consisted of at least two subunits. At the concentration of 1 CD50 (40 μg/mL) did facilitate transcytosis through the HUVEC cells monolayer of SEPEC 15 as much as E. coli K12 within 30 min without affecting cell viability. These results suggest that this cytotoxic factor, named as SPF (SEPEC's permeabilizing factor), may be an important SEPEC virulence factor that facilitates bacterial access to the bloodstream. |
Subject: | Citotoxinas Sepse Transcitose |
Country: | Reino Unido |
Editor: | Elsevier |
Rights: | Aberto |
Identifier DOI: | 10.1016/j.bjid.2016.01.003 |
Address: | https://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000300014 |
Date Issue: | 2016 |
Appears in Collections: | IB - Artigos e Outros Documentos |
Files in This Item:
File | Description | Size | Format | |
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S1413-86702016000300014.pdf | 1.18 MB | Adobe PDF | View/Open |
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