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Type: Outro documento
Title: Cytotoxic factor secreted by Escherichia coli associated with sepsis facilitates transcytosis through human umbilical vein endothelial cell monolayers
Author: Tibo, Luiz Henrique Soares
Bertol, Jéssica Wildgrube
Bernedo-Navarro, Robert Alvin
Yano, Tomomasa
Abstract: Culture supernatant of sepsis-associated Escherichia coli (SEPEC) isolated from patients with sepsis caused loss of intercellular junctions and elongation of human umbilical vein endothelial cells (HUVEC). The cytotoxic factor was purified from culture supernatant of SEPEC 15 (serogroup O153) by liquid chromatography process. PAGE (polyacrylamide gel electrophoresis) showed that the purified SEPEC cytotoxic factor had a molecular mass of ∼150 kDa and consisted of at least two subunits. At the concentration of 1 CD50 (40 μg/mL) did facilitate transcytosis through the HUVEC cells monolayer of SEPEC 15 as much as E. coli K12 within 30 min without affecting cell viability. These results suggest that this cytotoxic factor, named as SPF (SEPEC's permeabilizing factor), may be an important SEPEC virulence factor that facilitates bacterial access to the bloodstream.
Subject: Transcitose
Country: Reino Unido
Editor: Elsevier
Rights: Aberto
Identifier DOI: 10.1016/j.bjid.2016.01.003
Date Issue: 2016
Appears in Collections:IB - Artigos e Outros Documentos

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