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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampNociti Junior, Francisco Humberto-
dc.typeArtigopt_BR
dc.titleCounter-regulatory phosphatases TNAP and NPP1 temporally regulate tooth root cementogenesispt_BR
dc.contributor.authorZweifler, Laura E-
dc.contributor.authorPatel, Mudita K-
dc.contributor.authorNociti Jr, Francisco H-
dc.contributor.authorWimer, Helen F-
dc.contributor.authorMillán, Jose L-
dc.contributor.authorSomerman, Martha J-
dc.contributor.authorFoster, Brian L-
dc.subjectFosfatasespt_BR
dc.subject.otherlanguagePhosphatasespt_BR
dc.description.abstractCementum is critical for anchoring the insertion of periodontal ligament fibers to the tooth root. Several aspects of cementogenesis remain unclear, including differences between acellular cementum and cellular cementum, and between cementum and bone. Biomineralization is regulated by the ratio of inorganic phosphate (Pi) to mineral inhibitor pyrophosphate (PPi), where local Pi and PPi concentrations are controlled by phosphatases including tissue-nonspecific alkaline phosphatase (TNAP) and ectonucleotide pyrophosphatase/phosphodiesterase 1 (NPP1). The focus of this study was to define the roles of these phosphatases in cementogenesis. TNAP was associated with earliest cementoblasts near forming acellular and cellular cementum. With loss of TNAP in the Alpl null mouse, acellular cementum was inhibited, while cellular cementum production increased, albeit as hypomineralized cementoid. In contrast, NPP1 was detected in cementoblasts after acellular cementum formation, and at low levels around cellular cementum. Loss of NPP1 in the Enpp1 null mouse increased acellular cementum, with little effect on cellular cementum. Developmental patterns were recapitulated in a mouse model for acellular cementum regeneration, with early TNAP expression and later NPP1 expression. In vitro, cementoblasts expressed Alpl gene/protein early, whereas Enpp1 gene/protein expression was significantly induced only under mineralization conditions. These patterns were confirmed in human teeth, including widespread TNAP, and NPP1 restricted to cementoblasts lining acellular cementum. These studies suggest that early TNAP expression creates a low PPi environment promoting acellular cementum initiation, while later NPP1 expression increases PPi, restricting acellular cementum apposition. Alterations in PPi have little effect on cellular cementum formation, though matrix mineralization is affectedpt_BR
dc.relation.ispartofInternational journal of oral sciencept_BR
dc.relation.ispartofabbreviationInt J Oral Scipt_BR
dc.publisher.cityChengdupt_BR
dc.publisher.countryChinapt_BR
dc.publisherSichuan University Presspt_BR
dc.date.issued2015-
dc.language.isoengpt_BR
dc.description.volume7pt_BR
dc.description.firstpage27pt_BR
dc.description.lastpage41pt_BR
dc.rightsFechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn1674-2818pt_BR
dc.identifier.doi10.1038/ijos.2014.62pt_BR
dc.identifier.urlhttps://www.nature.com/articles/ijos201462pt_BR
dc.date.available2021-01-22T13:08:28Z-
dc.date.accessioned2021-01-22T13:08:28Z-
dc.description.provenanceSubmitted by Susilene Barbosa da Silva (susilene@unicamp.br) on 2021-01-22T13:08:28Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-02-26T13:23:15Z : No. of bitstreams: 1 000351189700006.pdf: 17477775 bytes, checksum: 839af38ec3c34fe8e81326c49047859a (MD5)en
dc.description.provenanceMade available in DSpace on 2021-01-22T13:08:28Z (GMT). No. of bitstreams: 0 Previous issue date: 2015en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/354599-
dc.contributor.departmentDepartamento de Prótese e Periodontiapt_BR
dc.contributor.unidadeFaculdade de Odontologia de Piracicabapt_BR
dc.subject.keywordCementogenesispt_BR
dc.identifier.source000351189700006pt_BR
dc.creator.orcid0000-0001-6809-8866pt_BR
dc.type.formArtigopt_BR
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