Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/354355
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampDa Silva, Livia Pilatti Mendes-
dc.contributor.authorunicampPaiva, Jacqueline Boldrin de-
dc.contributor.authorunicampRojas, Thaís Cabrera Galvão-
dc.contributor.authorunicampGarbim, Janaina Luisa Leite-
dc.contributor.authorunicampConceição, Rogério Arcuri-
dc.contributor.authorunicampDias da Silveira, Wanderley-
dc.typeArtigopt_BR
dc.titleThe virulence factor ychO has a pleiotropic action in an Avian Pathogenic Escherichia coli (APEC) strainpt_BR
dc.contributor.authorPilatti, Livia-
dc.contributor.authorPaiva, Jacqueline Boldrin de-
dc.contributor.authorRojas, Thaís Cabrera Galvão-
dc.contributor.authorLeite, Janaína Luisa-
dc.contributor.authorConceição, Rogério Arcuri-
dc.contributor.authorNakazato, Gerson-
dc.contributor.authorSilveira, Wanderley Dias da-
dc.subjectVirulenciapt_BR
dc.subjectEscherichia coli - Patogenicidadept_BR
dc.subject.otherlanguageEscherichia coli - Pathogenicitypt_BR
dc.subject.otherlanguageVirulencept_BR
dc.description.abstractAvian pathogenic Escherichia coli strains cause extraintestinal diseases in birds, leading to substantial economic losses to the poultry industry worldwide. Bacteria that invade cells can overcome the host humoral immune response, resulting in a higher pathogenicity potential. Invasins are members of a large family of outer membrane proteins that allow pathogen invasion into host cells by interacting with specific receptors on the cell surface. An in silico analysis of the genome of a septicemic APEC strain (SEPT362) demonstrated the presence of a putative invasin homologous to the ychO gene from E. coli str. K-12 substr. MG1655. In vitro and in vivo assays comparing a mutant strain carrying a null mutation of this gene, a complemented strain, and its counterpart wild-type strain showed that ychO plays a role in the pathogenicity of APEC strain SEPT362. In vitro assays demonstrated that the mutant strain exhibited significant decreases in bacterial adhesiveness and invasiveness in chicken cells and biofilm formation. In vivo assay indicated a decrease in pathogenicity of the mutant strain. Moreover, transcriptome analysis demonstrated that the ychO deletion affected the expression of 426 genes. Among the altered genes, 93.66 % were downregulated in the mutant, including membrane proteins and metabolism genes. The results led us to propose that gene ychO contributes to the pathogenicity of APEC strain SEPT362 influencing, in a pleiotropic manner, many biological characteristics, such as adhesion and invasion of in vitro cultured cells, biofilm formation and motility, which could be due to the possible membrane location of this protein. All of these results suggest that the absence of gene ychO would influence the virulence of the APEC strain herein studied.pt_BR
dc.relation.ispartofBMC microbiologypt_BR
dc.relation.ispartofabbreviationBMC microbiol.pt_BR
dc.publisher.cityLondonpt_BR
dc.publisher.countryReino Unidopt_BR
dc.publisherSpringer Naturept_BR
dc.date.issued2016-
dc.date.monthofcirculationMar.pt_BR
dc.language.isoengpt_BR
dc.description.volume16pt_BR
dc.description.issuenumber35pt_BR
dc.rightsAbertopt_BR
dc.sourceWOSpt_BR
dc.identifier.eissn1471-2180pt_BR
dc.identifier.doi10.1186/s12866-016-0654-2pt_BR
dc.identifier.urlhttps://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-016-0654-2pt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsordocumentnumberSem informaçãopt_BR
dc.description.sponsordocumentnumber2012/04391-1; 2012/09655-7pt_BR
dc.date.available2021-01-20T14:56:51Z-
dc.date.accessioned2021-01-20T14:56:51Z-
dc.description.provenanceSubmitted by Bruna Maria Campos da Cunha (bcampos@unicamp.br) on 2021-01-20T14:56:51Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-02-19T18:02:01Z : No. of bitstreams: 1 000372099500003.pdf: 1159959 bytes, checksum: 815dfb32d6f4cdc3958000d296a4ff1c (MD5)en
dc.description.provenanceMade available in DSpace on 2021-01-20T14:56:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2016en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/354355-
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentDepartamento de Genética, Evolução, Microbiologia e Imunologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.subject.keywordAPECpt_BR
dc.subject.keywordychOpt_BR
dc.identifier.source000372099500003pt_BR
dc.creator.orcid0000-0003-4306-5413pt_BR
dc.creator.orcid0000-0002-4844-0895pt_BR
dc.creator.orcidSem informaçãopt_BR
dc.creator.orcid0000-0002-0909-3296pt_BR
dc.creator.orcidSem informaçãopt_BR
dc.creator.orcid0000-0003-3550-0745pt_BR
dc.type.formArtigo de pesquisapt_BR
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