Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/354353
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampSantiago, André da Silva-
dc.typeArtigopt_BR
dc.titleProtein profile and protein interaction network of Moniliophthora perniciosa basidiosporespt_BR
dc.contributor.authorMares, Joise Hander-
dc.contributor.authorGramacho, Karina Peres-
dc.contributor.authorSantos, Everton Cruz dos-
dc.contributor.authorSantiago, André da Silva-
dc.contributor.authorSilva, Edson Mário de Andrade-
dc.contributor.authorAlvim, Fátima Cerqueira-
dc.contributor.authorPirovani, Carlos Priminho-
dc.subjectVassoura-de-bruxa (Fitopatologia)pt_BR
dc.subjectProteômicapt_BR
dc.subjectEspectrometria de massapt_BR
dc.subjectMoniliophthora perniciosapt_BR
dc.subject.otherlanguageMoniliophthora perniciosapt_BR
dc.subject.otherlanguageMass spectrometrypt_BR
dc.subject.otherlanguageProteomicspt_BR
dc.subject.otherlanguageWitches' broom diseasept_BR
dc.description.abstractWitches’ broom, a disease caused by the basidiomycete Moniliophthora perniciosa, is considered to be the most important disease of the cocoa crop in Bahia, an area in the Brazilian Amazon, and also in the other countries where it is found. M. perniciosa germ tubes may penetrate into the host through intact or natural openings in the cuticle surface, in epidermis cell junctions, at the base of trichomes, or through the stomata. Despite its relevance to the fungal life cycle, basidiospore biology has not been extensively investigated. In this study, our goal was to optimize techniques for producing basidiospores for protein extraction, and to produce the first proteomics analysis map of ungerminated basidiospores. We then presented a protein interaction network by using Ustilago maydis as a model. The average pileus area ranged from 17.35 to 211.24 mm2. The minimum and maximum productivity were 23,200 and 6,666,667 basidiospores per basidiome, respectively. The protein yield in micrograms per million basidiospores were approximately 0.161; 2.307, and 3.582 for germination times of 0, 2, and 4 h after germination, respectively. A total of 178 proteins were identified through mass spectrometry. These proteins were classified according to their molecular function and their involvement in biological processes such as cellular energy production, oxidative metabolism, stress, protein synthesis, and protein folding. Furthermore, to better understand the expression pattern, signaling, and interaction events of spore proteins, we presented an interaction network using orthologous proteins from Ustilago maydis as a model. Most of the orthologous proteins that were identified in this study were not clustered in the network, but several of them play a very important role in hypha development and branching. The quantities of basidiospores 7 × 109; 5.2 × 108, and 6.7 × 108 were sufficient to obtain enough protein mass for the three 2D-PAGE replicates, for the 0, 2, and 4 h-treatments, respectively. The protein extraction method that is based on sedimentation, followed by sonication with SDS-dense buffer, and phenolic extraction, which was utilized in this study, was effective, presenting a satisfactory resolution and reproducibility for M. perniciosa basidiospores. This report constitutes the first comprehensive study of protein expression during the ungerminated stage of the M. perniciosa basidiospore. Identification of the spots observed in the reference gel enabled us to know the main molecular interactions involved in the initial metabolic processes of fungal development.pt_BR
dc.relation.ispartofBMC microbiologypt_BR
dc.relation.ispartofabbreviationBMC microbiol.pt_BR
dc.publisher.cityLondonpt_BR
dc.publisher.countryReino Unidopt_BR
dc.publisherSpringer Naturept_BR
dc.date.issued2016-
dc.date.monthofcirculationJunept_BR
dc.language.isoengpt_BR
dc.description.volume16pt_BR
dc.description.issuenumber120pt_BR
dc.rightsAbertopt_BR
dc.sourceWOSpt_BR
dc.identifier.eissn1471-2180pt_BR
dc.identifier.doi10.1186/s12866-016-0753-0pt_BR
dc.identifier.urlhttps://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-016-0753-0pt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DA BAHIA - FAPESBpt_BR
dc.description.sponsordocumentnumber305309/2012-9pt_BR
dc.description.sponsordocumentnumberPNE0005/2011pt_BR
dc.date.available2021-01-20T14:50:23Z-
dc.date.accessioned2021-01-20T14:50:23Z-
dc.description.provenanceSubmitted by Bruna Maria Campos da Cunha (bcampos@unicamp.br) on 2021-01-20T14:50:23Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-02-19T18:02:01Z : No. of bitstreams: 1 000378857600001.pdf: 2267096 bytes, checksum: 2855f3f59a686aa66b7fa7c27ecacf46 (MD5)en
dc.description.provenanceMade available in DSpace on 2021-01-20T14:50:23Z (GMT). No. of bitstreams: 0 Previous issue date: 2016en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/354353-
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.subject.keywordBasidiosporespt_BR
dc.identifier.source000378857600001pt_BR
dc.creator.orcidSem informaçãopt_BR
dc.type.formArtigo de pesquisapt_BR
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