Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/354342
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampDhyani, Anamika-
dc.contributor.authorunicampSaad, Sara Teresinha Olalla-
dc.typeArtigopt_BR
dc.titleANKHD1 is an S phase protein required for histone synthesis and DNA repair in multiple myeloma cellspt_BR
dc.contributor.authorDhyani, Anamika-
dc.contributor.authorFavaro, Patricia-
dc.contributor.authorSaad, Sara T Olalla-
dc.subjectHistonaspt_BR
dc.subject.otherlanguageHistonespt_BR
dc.description.abstractANKHD1 is highly expressed in various cancers such as leukemia and multiple myeloma. Silencing of ANKHD1 expression leads to decreased cell proliferation and accumulation of cells at the S phase. In this study we found ANKHD1 expression to be higher at the S phase, suggesting it to be an S phase protein. We observed that ANKHD1 interacts with histone promoter regions and its inhibition downregulates expression of all core histones, implying a role in histone synthesis. Since histone synthesis occurs in parallel with DNA replication at S phase, we evaluated PCNA (Proliferating Cell Nuclear Antigen) expression, a protein involved in DNA replication and repair. PCNA expression was found to be significantly decreased in ANKHD1 silenced cells. We further observed accumulation γH2AX, a marker for DNA double stranded breaks and an early sign of DNA damage induced by replication stress, upon ANKHD1 silencing. The expressions of several genes implicated in DNA repair were also modulated in ANKHD1 silenced cells, confirming the role of ANKHD1 in DNA repair. Based on this study we speculate that ANKHD1 is an S phase protein required for histone synthesis and DNA repair. These results however, are preliminary and require thorough investigationpt_BR
dc.relation.ispartofBlood cells, molecules and diseasespt_BR
dc.relation.ispartofabbreviationBlood cells mol. dis.pt_BR
dc.publisher.cityAmsterdampt_BR
dc.publisher.countryPaíses Baixospt_BR
dc.publisherElsevierpt_BR
dc.date.issued2020-
dc.date.monthofcirculationSept.pt_BR
dc.language.isoengpt_BR
dc.description.volume84pt_BR
dc.rightsFechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn1079-9796pt_BR
dc.identifier.eissn1096-0961pt_BR
dc.identifier.doi10.1016/j.bcmd.2020.102460pt_BR
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S1079979620301315pt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumberSem informaçãopt_BR
dc.description.sponsordocumentnumberSem informaçãopt_BR
dc.date.available2021-01-20T13:25:25Z-
dc.date.accessioned2021-01-20T13:25:25Z-
dc.description.provenanceSubmitted by Thais de Brito Barroso (tbrito@unicamp.br) on 2021-01-20T13:25:25Z No. of bitstreams: 0en
dc.description.provenanceMade available in DSpace on 2021-01-20T13:25:25Z (GMT). No. of bitstreams: 0 Previous issue date: 2020en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/354342-
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentDepartamento de Clínica Médicapt_BR
dc.contributor.unidadeCentro de Hematologia e Hemoterapiapt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.subject.keywordAnkyrin repeat and KH domain-containing 1pt_BR
dc.subject.keywordS phasept_BR
dc.subject.keywordProliferating cell nuclear antigenpt_BR
dc.subject.keywordDNA damagept_BR
dc.subject.keywordMyelomapt_BR
dc.identifier.source000548779400011pt_BR
dc.creator.orcidSem informaçãopt_BR
dc.creator.orcid0000-0003-0809-8068pt_BR
dc.type.formArtigopt_BR
dc.identifier.articleid102460pt_BR
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