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Type: Artigo
Title: Different cell death responses induced by Eupomatenoid-5 in MCF-7 and 786-0 tumor cell lines
Author: Longato, Giovanna Barbarini
Fiorito, Giovanna Francisco
Vendramini-Costa, Debora Barbosa
de Oliveira Sousa, Ilza Maria
Tinti, Sirlene Valerio
Tasca Gois Ruiz, Ana Lucia
Vitalino de Almeida, Sinara Monica
Ribeiro Padilha, Rafael Jose
Foglio, Mary Ann
de Carvalho, Joao Ernesto
Abstract: Natural products remain an important source of new drugs, including anticancer drugs. Recently, our group reported the anticancer activity of eupomatenoid-5 (eup-5), a neolignan isolated from Piper regnellii (Miq.) C. DC. var. regnellii leaves. In vitro studies demonstrated that MCF-7 (breast) and 786-0 (kidney) were among the cancer cell lines most sensitive to eup-5 treatment. The current results demonstrate that mitochondrial membrane depolarization and generation of reactive oxygen species are implicated in eup-5-mediated cytotoxic effects on these cancer cells lines. In MCF-7 cells, eup-5 led to phosphatidylserine externalization and caspase activation, whereas the same did not occur in 786-0 cells. Scanning electron microscopy revealed a reduction of microvilli density, as well as cell morphology alterations. Moreover, treated MCF-7 cells exhibited well-characterized apoptosis alterations, while treated 786-0 cells exhibited characteristics of programmed necroptosis process. These findings support the possibility that different mechanisms may be targeted by eup-5 in cell death response
Subject: Apoptose
Country: Reino Unido
Editor: Elsevier
Rights: Fechado
Identifier DOI: 10.1016/j.tiv.2015.04.002
Date Issue: 2015
Appears in Collections:IB - Artigos e Outros Documentos
CPQBA - Artigos e Outros Documentos
FCF - Artigos e Outros Documentos

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