Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/353419
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampCarvalho Filho, Élio Barreto-
dc.contributor.authorunicampMarson, Fernando Augusto de Lima-
dc.contributor.authorunicampAngerami, Rodrigo Nogueira-
dc.contributor.authorunicampLevy, Carlos Emilio-
dc.typeArtigopt_BR
dc.titleEvaluation of PCR in the diagnosis of Canine Leishmaniasis in two different epidemiological regions: Campinas (sp) and Teresina (pi), Brazilpt_BR
dc.contributor.authorCosta, L. N. G.-
dc.contributor.authorBorba, A. S.-
dc.contributor.authorCastagna, C. L.-
dc.contributor.authorCarvalho Filho, E. B.-
dc.contributor.authorMarson, F. A. L.-
dc.contributor.authorSa Junior, F. F.-
dc.contributor.authorAngerami, R. N.-
dc.contributor.authorLevy, C. E.-
dc.subjectLeishmaniosept_BR
dc.subjectEpidemiologiapt_BR
dc.subject.otherlanguageLeishmaniasispt_BR
dc.subject.otherlanguageEpidemiologypt_BR
dc.description.abstractUsing the polymerase chain reaction (PCR) test for diagnosis of canine leishmaniasis has greater sensitivity and specificity than culture and visualization of the parasite. This study compares PCR for the diagnosis of the genus and species of Leishmania with serological techniques used for the control of canine visceral leishmaniasis (CVL) in Brazil, considering two regions. We analysed peripheral blood samples collected from 195 dogs in the Campinas (SP) and Teresina (PI) regions. ELISA was performed as a serological method and PCR was performed using specific primers for the genus Leishmania spp. and the species Leishmania chagasi. In Campinas, a greater sensitivity of PCR (88.24%) (P=0.0455) compared to Teresina (14.71%) (P<0.0001) was observed, and an agreement was observed for Cohen's kappa index (0.9096). Both PCR and ELISA showed discordance for sensitivity (Campinas 100%, Teresina 21.74%), specificity (Campinas 30.77%, Teresina 100%), positive predictive value (Campinas 68.97%, Teresina 100%), negative predictive value (Campinas 100%, Teresina 37.94%) and Cohen's kappa index (0.1238). This study confirms the importance of PCR in analysis of the canine reservoir, and as an effective method for the detection of active and recent infectionpt_BR
dc.relation.ispartofEpidemiology and Infectionpt_BR
dc.relation.ispartofabbreviationEpidemiol. Infect.pt_BR
dc.publisher.cityCambridgept_BR
dc.publisher.countryReino Unidopt_BR
dc.publisherCambridge University Presspt_BR
dc.date.issued2015-
dc.date.monthofcirculationApr.pt_BR
dc.language.isoengpt_BR
dc.description.volume143pt_BR
dc.description.issuenumber5pt_BR
dc.description.firstpage1088pt_BR
dc.description.lastpage1095pt_BR
dc.rightsAbertopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn0950-2688pt_BR
dc.identifier.eissn1469-4409pt_BR
dc.identifier.doi10.1017/S0950268814001721pt_BR
dc.identifier.urlhttps://www.cambridge.org/core/journals/epidemiology-and-infection/article/evaluation-of-pcr-in-the-diagnosis-of-canine-leishmaniasis-in-two-different-epidemiological-regions-campinas-sp-and-teresina-pi-brazil/2E8272F44779AC1FC36E786C8BBEF6A3pt_BR
dc.date.available2021-01-04T12:22:55Z-
dc.date.accessioned2021-01-04T12:22:55Z-
dc.description.provenanceSubmitted by Cintia Oliveira de Moura (cintiaom@unicamp.br) on 2021-01-04T12:22:55Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-02-19T18:59:58Z : No. of bitstreams: 1 000351059300025.pdf: 212152 bytes, checksum: 8cefd0e01c567e39aab931ccbcfbc6a9 (MD5)en
dc.description.provenanceMade available in DSpace on 2021-01-04T12:22:55Z (GMT). No. of bitstreams: 0 Previous issue date: 2015en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/353419-
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentDepartamento de Patologia Clínicapt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.identifier.source000351059300025pt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcid0000-0003-4955-4234pt_BR
dc.creator.orcid0000-0002-4976-9238pt_BR
dc.creator.orcid0000-0002-4862-8900pt_BR
dc.type.formArtigopt_BR
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