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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampAparicio, Ricardo-
dc.typeArtigopt_BR
dc.titleInsights into the structure and function of fungal β‐mannosidases from glycoside hydrolase family 2 based on multiple crystal structures of the Trichoderma harzianum enzymept_BR
dc.contributor.authorNascimento, Alessandro S.-
dc.contributor.authorMuniz, Joao Renato C.-
dc.contributor.authorAparício, Ricardo-
dc.contributor.authorGolubev, Alexander M.-
dc.contributor.authorPolikarpov, Igor-
dc.subjectGlicosídeo hidrolasespt_BR
dc.subject.otherlanguageGlycoside hydrolasespt_BR
dc.description.abstractHemicellulose is an important part of the plant cell wall biomass, and is relevant to cellulosic ethanol technologies. β‐Mannosidases are enzymes capable of cleaving nonreducing residues of β‐d‐mannose from β‐d‐mannosides and hemicellulose mannose‐containing polysaccharides, such as mannans and galactomannans. β‐Mannosidases are distributed between glycoside hydrolase (GH) families 1, 2, and 5, and only a handful of the enzymes have been structurally characterized to date. The only published X‐ray structure of a GH family 2 mannosidase is that of the bacterial Bacteroides thetaiotaomicron enzyme. No structures of eukaryotic mannosidases of this family are currently available. To fill this gap, we set out to solve the structure of Trichoderma harzianum GH family 2 β‐mannosidase and to refine it to 1.9‐Å resolution. Structural comparisons of the T. harzianum GH2 β‐mannosidase highlight similarities in its structural architecture with other members of GH family 2, reveal the molecular mechanism of β‐mannoside binding and recognition, and shed light on its putative galactomannan‐binding sitept_BR
dc.relation.ispartofThe FEBS journalpt_BR
dc.relation.ispartofabbreviationFEBS j.pt_BR
dc.publisher.cityChichesterpt_BR
dc.publisher.countryReino Unidopt_BR
dc.publisherWileypt_BR
dc.date.issued2014-
dc.date.monthofcirculationSep.pt_BR
dc.language.isoengpt_BR
dc.description.volume281pt_BR
dc.description.issuenumber18pt_BR
dc.description.firstpage4165pt_BR
dc.description.lastpage4178pt_BR
dc.rightsFechadopt_BR
dc.sourceSCOPUSpt_BR
dc.identifier.issn1742-464Xpt_BR
dc.identifier.eissn1742-4658pt_BR
dc.identifier.doi10.1111/febs.12894pt_BR
dc.identifier.urlhttps://febs.onlinelibrary.wiley.com/doi/full/10.1111/febs.12894pt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsorshipCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumber490022/2009‐0; 301981/2011‐6; 400045/2012‐5; 485950/2013‐8pt_BR
dc.description.sponsordocumentnumbersem informaçãopt_BR
dc.description.sponsordocumentnumber2008/56255‐9; 2009/52840‐7pt_BR
dc.date.available2020-12-21T18:05:11Z-
dc.date.accessioned2020-12-21T18:05:11Z-
dc.description.provenanceSubmitted by Sanches Olivia (olivias@unicamp.br) on 2020-12-21T18:05:11Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-02-25T19:55:38Z : No. of bitstreams: 1 2-s2.0-84909955528.pdf: 1761314 bytes, checksum: 5724b8624ae71fd3045248dea5538e84 (MD5)en
dc.description.provenanceMade available in DSpace on 2020-12-21T18:05:11Z (GMT). No. of bitstreams: 0 Previous issue date: 2014en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/353350-
dc.contributor.departmentDepartamento de Físico-Químicapt_BR
dc.contributor.unidadeInstituto de Químicapt_BR
dc.subject.keywordCrystal structurept_BR
dc.subject.keywordGalactomannan bindingpt_BR
dc.subject.keywordGlycosyl hydrolase family 2pt_BR
dc.subject.keywordTrichoderma harzianumpt_BR
dc.subject.keywordβ-mannosidasept_BR
dc.identifier.source2-s2.0-84909955528pt_BR
dc.creator.orcidsem informaçãopt_BR
dc.type.formArtigo originalpt_BR
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