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Type: Artigo
Title: Chitosan-tripolyphosphate nanoparticles as arrabidaea chica standardized extract carrier: synthesis, characterization, biocompatibility, and antiulcerogenic activity
Author: Servat-Medina, Leila
Gonzalez-Gomez, Alvaro
Reyes-Ortega, Felisa
Oliveira Sousa, Ilza Maria
Almeida Queiroz, Nubia de Cassia
Wiziack Zago, Patricia Maria
orge, Michelle Pedrosa
Monteiro, Karin Maia
de Carvalho, Joao Ernesto
San Roman, Julio
Foglio, Mary Ann
Abstract: Natural products using plants have received considerable attention because of their potential to treat various diseases. Arrabidaea chica (Humb. & Bonpl.) B. Verlot is a native tropical American vine with healing properties employed in folk medicine for wound healing, inflammation, and gastrointestinal colic. Applying nanotechnology to plant extracts has revealed an advantageous strategy for herbal drugs considering the numerous features that nanostructured systems offer, including solubility, bioavailability, and pharmacological activity enhancement. The present study reports the preparation and characterization of chitosan-sodium tripolyphosphate nanoparticles (NPs) charged with A. chica standardized extract (AcE). Particle size and zeta potential were measured using a Zetasizer Nano ZS. The NP morphological characteristics were observed using scanning electron microscopy. Our studies indicated that the chitosan/sodium tripolyphosphate mass ratio of 5 and volume ratio of 10 were found to be the best condition to achieve the lowest NP sizes, with an average hydrodynamic diameter of 150 +/- 13 nm and a zeta potential of +45 +/- 2 mV. Particle size decreased with AcE addition (60 +/- 10.2 nm), suggesting an interaction between the extract's composition and polymers. The NP biocompatibility was evaluated using human skin fibroblasts. AcE-NP demonstrated capability of maintaining cell viability at the lowest concentrations tested, stimulating cell proliferation at higher concentrations. Antiulcerogenic activity of AcE-NP was also evaluated with an acute gastric ulcer experimental model induced by ethanol and indomethacin. NPs loaded with A. chica extract reduced the ulcerative lesion index using lower doses compared with the free extract, suggesting that extract encapsulation in chitosan NPs allowed for a dose reduction for a gastroprotective effect. The AcE encapsulation offers an approach for further application of the A. chica extract that could be considered a potential candidate for ulcer-healing pharmaceutical systems
Subject: Arrabidaea chica
Country: Reino Unido
Editor: Dove Medical
Rights: Fechado
Identifier DOI: 10.2147/IJN.S83705
Date Issue: 2015
Appears in Collections:IB - Artigos e Outros Documentos
FCM - Artigos e Outros Documentos
CPQBA - Artigos e Outros Documentos
FOP - Artigos e Outros Documentos

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