Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/353227
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampNobrega, Letícia Maria Menezes-
dc.contributor.authorunicampMontagner, Francisco-
dc.contributor.authorunicampGomes, Brenda Paula Figueiredo de Almeida-
dc.typeArtigopt_BR
dc.titleMolecular identification of cultivable bacteria from infected root canals associated with acute apical abscesspt_BR
dc.contributor.authorNóbrega, Letícia M. M.-
dc.contributor.authorMontagner, Francisco-
dc.contributor.authorRibeiro, Adriana C.-
dc.contributor.authorMayer, Márcia A. P.-
dc.contributor.authorGomes, Brenda P. F. A.-
dc.subjectEndodontiapt_BR
dc.subject.otherlanguageEndodonticspt_BR
dc.description.abstractThe objective of this study was to investigate the bacterial composition present in root canals of teeth associated with acute apical abscess by molecular identification (16S rRNA) of cultivable bacteria. Two hundred and twenty strains isolated by culture from 20 root canals were subjected to DNA extraction and amplification of the 16S rRNA gene (PCR), followed by sequencing. The resulting nucleotide sequences were compared to the GenBank database from the National Center of Biotechnology Information through BLAST. Strains not identified by sequencing were submitted to clonal analysis. The association of microbiological findings with clinical features and the association between microbial species were also investigated. Fifty-nine different cultivable bacteria were identified by 16S rRNA gene sequencing, belonging to 6 phyla, with an average number of 6 species per root canal. Molecular approaches allowed identification of 99% of isolates. The most frequently identified bacteria were Prevotella spp., Pseudoramibacter alactolyticus, Parvimonas micra, Dialister invisus, Filifactor alocis, and Peptostreptococcus stomatis. Positive association was found between Prevotella buccae and Pseudoramibacter alactolyticus and between Parvimonas micra and Prevotella nigrescens (both p<0.05). It was concluded that the microbiota of infected root canals associated with acute apical abscess is diverse and heterogeneous, composed mainly of anaerobic Gram-negative bacteria, with the great majority belonging to the phyla Firmicutes and Bacteroidetespt_BR
dc.relation.ispartofBrazilian dental journalpt_BR
dc.relation.ispartofabbreviationBraz dent jpt_BR
dc.publisher.cityRibeirão Preto, SPpt_BR
dc.publisher.countryBrasilpt_BR
dc.publisherUSP/Fundação Odontológica de Ribeirão Pretopt_BR
dc.date.issued2016-
dc.date.monthofcirculationMay/Junept_BR
dc.language.isoengpt_BR
dc.description.volume27pt_BR
dc.description.issuenumber3pt_BR
dc.description.firstpage318pt_BR
dc.description.lastpage324pt_BR
dc.rightsAbertopt_BR
dc.sourceSciELOpt_BR
dc.identifier.issn0103-6440pt_BR
dc.identifier.eissn1806-4760pt_BR
dc.identifier.doi10.1590/0103-6440201600715pt_BR
dc.identifier.urlhttps://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402016000300318pt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsorshipCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumber302575/2009; 308162/2014-5pt_BR
dc.description.sponsordocumentnumberSem informaçãopt_BR
dc.description.sponsordocumentnumber2009/07760-5; 2011/09047-4pt_BR
dc.date.available2020-12-17T13:58:16Z-
dc.date.accessioned2020-12-17T13:58:16Z-
dc.description.provenanceSubmitted by Thais de Brito Barroso (tbrito@unicamp.br) on 2020-12-17T13:58:16Z No. of bitstreams: 0en
dc.description.provenanceMade available in DSpace on 2020-12-17T13:58:16Z (GMT). No. of bitstreams: 0 Previous issue date: 2016en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/353227-
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentDepartamento de Odontologia Restauradorapt_BR
dc.contributor.unidadeFaculdade de Odontologia de Piracicabapt_BR
dc.contributor.unidadeFaculdade de Odontologia de Piracicabapt_BR
dc.contributor.unidadeFaculdade de Odontologia de Piracicabapt_BR
dc.description.abstractalternativeO objetivo deste estudo foi investigar a composição bacteriana de canais radiculares associados com abscesso apical agudo através de identificação molecular (16S rRNA) de bactérias cultiváveis. Duzentas e vinte cepas, de 20 casos, isoladas por cultura foram submetidas a extração de DNA e amplificação do gene 16S rRNA (PCR), seguido de sequenciamento. As sequências de nucleotídeos obtidas foram comparadas com o banco de dados (GenBank) do National Center of Biotechnology Information através do BLAST. Cepas não identificadas pelo sequenciamento foram submetidas à clonagem. Associação de achados microbiológicos e características clínicas e associação entre espécies bacterianas também foram investigadas. Cinquenta e nove bactérias cultiváveis diferentes foram identificadas pelo sequenciamento do gene 16S rRNA, pertencentes a 6 filos, numa média de 6 espécies por canal. Método molecular permitiu identificação de 99% das cepas isoladas. As bactérias mais frequentes foram Prevotella spp., Pseudoramibacter alactolyticus, Parvimonas micra, Dialister invisus, Filifactor alocis, Peptostreptococcus stomatis. Associação positiva foi encontrada entre Prevotella buccae e Pseudoramibacter alactolyticus, e entre Parvimonas micra e Prevotella nigrescens (p<0,05). Foi concluído que a microbiota de canais radiculares infectados associados com abscesso apical agudo é diversa e heterogênea, composta principalmente por anaeróbios Gram-negativos, pertencentes aos filos Firmicutes e Bacteroidetespt_BR
dc.subject.keywordRoot canalspt_BR
dc.subject.keywordMicroorganismspt_BR
dc.subject.keywordAbscesspt_BR
dc.subject.keywordSequencingpt_BR
dc.identifier.sourceS0103-64402016000300318pt_BR
dc.creator.orcidSem informaçãopt_BR
dc.creator.orcid0000-0002-7850-0107pt_BR
dc.creator.orcid0000-0002-8449-0646pt_BR
dc.type.formArtigopt_BR
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