Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/352894
Type: Artigo
Title: Virulence and pathogenicity of candida albicans is enhanced in biofilms containing oral bacteria
Author: Cavalcanti, Yuri Wanderley
Morse, Daniel James
da Silva, Wander Jose
Del-Bel-Cury, Altair Antoninha
Wei, Xiaoqing
Wilson, Melanie
Milward, Paul
Lewis, Michael
Lewis, Michael
Williams, David Wynne
Abstract: This study examined the influence of bacteria on the virulence and pathogenicity of candidal biofilms. Mature biofilms (Candida albicans-only, bacteria-only, C. albicans with bacteria) were generated on acrylic and either analysed directly, or used to infect a reconstituted human oral epithelium (RHOE). Analyses included Candida hyphae enumeration and assessment of Candida virulence gene expression. Lactate dehydrogenase (LDH) activity and Candida tissue invasion following biofilm infection of the RHOE were also measured. Candida hyphae were more prevalent (p < 0.05) in acrylic biofilms also containing bacteria, with genes encoding secreted aspartyl-proteinases (SAP4/SAP6) and hyphal-wall protein (HWP1) up-regulated (p < 0.05). Candida adhesin genes (ALS3/EPA1), SAP6 and HWP1 were up-regulated in mixed-species biofilm infections of RHOE. Multi-species infections exhibited higher hyphal proportions (p < 0.05), up-regulation of IL-18, higher LDH activity and tissue invasion. As the presence of bacteria in acrylic biofilms promoted Candida virulence, consideration should be given to the bacterial component when managing denture biofilm associated candidoses
Subject: Biofilmes
Candida albicans
Country: Reino Unido
Editor: Taylor & Francis
Rights: Aberto
Identifier DOI: 10.1080/08927014.2014.996143
Address: https://www.tandfonline.com/doi/full/10.1080/08927014.2014.996143
Date Issue: 2015
Appears in Collections:FOP - Artigos e Outros Documentos

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