Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/352216
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampLeite Júnior, Bruno Ricardo de Castro-
dc.contributor.authorunicampTribst, Alline Artigiani Lima-
dc.contributor.authorunicampCristianini, Marcelo-
dc.typeArtigopt_BR
dc.titleBiophysical evaluation of milk-clotting enzymes processed by high pressurept_BR
dc.contributor.authorLeite Júnior, Bruno Ricardo de Castro-
dc.contributor.authorTribst, Alline Artigiani Lima-
dc.contributor.authorGrant, Nicholas J.-
dc.contributor.authorYada, Rickey Y.-
dc.contributor.authorCristianini, Marcelo-
dc.subjectEnzimaspt_BR
dc.subject.otherlanguageEnzymespt_BR
dc.description.abstractHigh pressure processing (HPP) is able to promote changes in enzymes structure. This study evaluated the effect of HP on the structural changes in milk-clotting enzymes processed under activation conditions for recombinant camel chymosin (212 MPa/5 min/10 °C), calf rennet (280 MPa/20 min/25 °C), bovine rennet (222 MPa/5 min/23 °C), and porcine pepsin (50 MPa/5 min/20 °C) and under inactivation conditions for all enzymes (600 MPa/10 min/25 °C) including the protease from Rhizomucor miehei. In general, it was found that the HPP at activation conditions was able to increase the intrinsic fluorescence of samples with high pepsin concentration (porcine pepsin and bovine rennet), increase significantly the surface hydrophobicity and induce changes in secondary structure of all enzymes. Under inactivation conditions, increases in surface hydrophobicity and a reduction of intrinsic fluorescence were observed, suggesting a higher exposure of hydrophobic sites followed by water quenching of Trp residues. Moreover, changes in secondary structure were observed (with minor changes seen in Rhizomucor miehei protease). In conclusion, HPP was able to unfold milk-clotting enzymes even under activation conditions, and the porcine pepsin and bovine rennet were more sensitive to HPPpt_BR
dc.relation.ispartofFood research internationalpt_BR
dc.relation.ispartofabbreviationFood res. int.pt_BR
dc.publisher.cityOxfordpt_BR
dc.publisher.countryReino Unidopt_BR
dc.publisherElsevierpt_BR
dc.date.issued2017-
dc.date.monthofcirculationJulypt_BR
dc.language.isoengpt_BR
dc.description.volume97pt_BR
dc.description.firstpage116pt_BR
dc.description.lastpage122pt_BR
dc.rightsFechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn0963-9969pt_BR
dc.identifier.eissn1873-7145pt_BR
dc.identifier.doi10.1016/j.foodres.2017.03.042pt_BR
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S0963996917301424pt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumber2012/13509-6; 2014/17782-4; 2016/01425-3pt_BR
dc.date.available2020-11-12T00:40:43Z-
dc.date.accessioned2020-11-12T00:40:43Z-
dc.description.provenanceSubmitted by Thais de Brito Barroso (tbrito@unicamp.br) on 2020-11-12T00:40:43Z No. of bitstreams: 0en
dc.description.provenanceMade available in DSpace on 2020-11-12T00:40:43Z (GMT). No. of bitstreams: 0 Previous issue date: 2017en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/352216-
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentDepartamento de Tecnologia de Alimentospt_BR
dc.contributor.unidadeFaculdade de Engenharia de Alimentospt_BR
dc.contributor.unidadeNúcleo de Estudos e Pesquisas em Alimentaçãopt_BR
dc.contributor.unidadeFaculdade de Engenharia de Alimentospt_BR
dc.subject.keywordHigh pressure processingpt_BR
dc.subject.keywordMilk-clotting enzymespt_BR
dc.subject.keywordIntrinsic fluorescencept_BR
dc.subject.keywordSurface hydrophobicitypt_BR
dc.subject.keywordATR-FTIR spectroscopypt_BR
dc.identifier.source000403861500014pt_BR
dc.creator.orcid0000-0001-9030-2819pt_BR
dc.creator.orcid0000-0003-1946-9354pt_BR
dc.creator.orcid0000-0001-7740-0449pt_BR
dc.type.formArtigopt_BR
Appears in Collections:FEA - Artigos e Outros Documentos
NEPA - Artigos e Outros Documentos

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