Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/351409
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampDias, Fernanda Furlan Gonçalves-
dc.contributor.authorunicampRuiz, Ana Lucia Tasca Gois-
dc.contributor.authorunicampDella Torre, Adriana-
dc.contributor.authorunicampSato, Helia Harumi-
dc.typeArtigopt_BR
dc.titlePurification, characterization and antiproliferative activity of l-asparaginase from aspergillus oryzae CCT 3940 with no glutaminase activitypt_BR
dc.contributor.authorDias, Fernanda Furlan Gonçalves-
dc.contributor.authorRuiz, Ana Lúcia Tasca Gois-
dc.contributor.authorTorre, Adriana Della-
dc.contributor.authorSato, Helia Harumi-
dc.subjectAspergillus oryzaept_BR
dc.subject.otherlanguageAspergillus oryzaept_BR
dc.description.abstractTo explore the anti-proliferative activity of purified l-asparaginase from Aspergillus oryzae CCT 3940 (A. oryzae). Methods l-asparaginase was produced by submerged fermentation and purified to electrophoresis homogeneity by ionic exchanged chromatography in a fast protein liquid chromatographic system. The purified enzyme was characterized and used for the antiproliferative assay against nine tumor cell lines and one non-tumor cell line. Results The free glutaminase l-asparaginase was purified 28.6 fold. l-asparaginase showed high stability under physiological condition, remaining stable in the pH range 7.0–8.0 after 1 h incubation at temperature range 30–45 °C. The Km and Vmax values of purified l-asparaginase were estimated as 0.66 mmol/L and 313 IU/mL, respectively. The purified enzyme could inhibit the growth of a broad range of human tumor cell lines at the concentrations studied. Also, the enzyme from A. oryzae CCT 3940 could inhibit tumor growth of leukemia cell line (K562) with a total growth inhibition value of (3.2 ± 2.5) IU/mL and did not inhibit the non-carcinogenic human cell line growth at the concentrations studied. Conclusions The sensitivity of the cells lines to purified l-asparaginase from A. oryzae CCT 3940 appeared to be concentration dependent affording a more significant decrease in cell growth than that observed for the commercial l-asparaginase from Escherichia coli. The l-asparaginase from A. oryzae CCT 3940 has a high potential for pharmaceutical exploitation in the treatment of leukemiapt_BR
dc.relation.ispartofAsian pacific journal of tropical biomedicinept_BR
dc.publisher.cityMumbaipt_BR
dc.publisher.countryÍndiapt_BR
dc.publisherWolters Kluwerpt_BR
dc.date.issued2016-
dc.date.monthofcirculationSept.pt_BR
dc.language.isoengpt_BR
dc.description.volume6pt_BR
dc.description.issuenumber9pt_BR
dc.description.firstpage785pt_BR
dc.description.lastpage794pt_BR
dc.rightsAbertopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn2221-1691pt_BR
dc.identifier.eissn2588-9222pt_BR
dc.identifier.doi10.1016/j.apjtb.2016.07.007pt_BR
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S222116911630096Xpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumber2012/24046-7pt_BR
dc.date.available2020-10-22T16:38:40Z-
dc.date.accessioned2020-10-22T16:38:40Z-
dc.description.provenanceSubmitted by Thais de Brito Barroso (tbrito@unicamp.br) on 2020-10-22T16:38:40Z No. of bitstreams: 0en
dc.description.provenanceMade available in DSpace on 2020-10-22T16:38:40Z (GMT). No. of bitstreams: 0 Previous issue date: 2016en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/351409-
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentDepartamento de Ciência de Alimentospt_BR
dc.contributor.unidadeFaculdade de Engenharia de Alimentospt_BR
dc.contributor.unidadeFaculdade de Ciências Farmacêuticaspt_BR
dc.contributor.unidadeCentro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolaspt_BR
dc.subject.keywordL-asparaginasept_BR
dc.subject.keywordPurificationpt_BR
dc.subject.keywordAnti-tumoral activitypt_BR
dc.identifier.source000382292100012pt_BR
dc.creator.orcidSem informaçãopt_BR
dc.creator.orcid0000-0002-0844-8702pt_BR
dc.creator.orcidSem informaçãopt_BR
dc.creator.orcid0000-0002-8170-7088pt_BR
dc.type.formArtigo originalpt_BR
Appears in Collections:FEA - Artigos e Outros Documentos
CPQBA - Artigos e Outros Documentos
FCF - Artigos e Outros Documentos

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