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http://repositorio.unicamp.br/jspui/handle/REPOSIP/351409
Type: | Artigo |
Title: | Purification, characterization and antiproliferative activity of l-asparaginase from aspergillus oryzae CCT 3940 with no glutaminase activity |
Author: | Dias, Fernanda Furlan Gonçalves Ruiz, Ana Lúcia Tasca Gois Torre, Adriana Della Sato, Helia Harumi |
Abstract: | To explore the anti-proliferative activity of purified l-asparaginase from Aspergillus oryzae CCT 3940 (A. oryzae). Methods l-asparaginase was produced by submerged fermentation and purified to electrophoresis homogeneity by ionic exchanged chromatography in a fast protein liquid chromatographic system. The purified enzyme was characterized and used for the antiproliferative assay against nine tumor cell lines and one non-tumor cell line. Results The free glutaminase l-asparaginase was purified 28.6 fold. l-asparaginase showed high stability under physiological condition, remaining stable in the pH range 7.0–8.0 after 1 h incubation at temperature range 30–45 °C. The Km and Vmax values of purified l-asparaginase were estimated as 0.66 mmol/L and 313 IU/mL, respectively. The purified enzyme could inhibit the growth of a broad range of human tumor cell lines at the concentrations studied. Also, the enzyme from A. oryzae CCT 3940 could inhibit tumor growth of leukemia cell line (K562) with a total growth inhibition value of (3.2 ± 2.5) IU/mL and did not inhibit the non-carcinogenic human cell line growth at the concentrations studied. Conclusions The sensitivity of the cells lines to purified l-asparaginase from A. oryzae CCT 3940 appeared to be concentration dependent affording a more significant decrease in cell growth than that observed for the commercial l-asparaginase from Escherichia coli. The l-asparaginase from A. oryzae CCT 3940 has a high potential for pharmaceutical exploitation in the treatment of leukemia |
Subject: | Aspergillus oryzae |
Country: | Índia |
Editor: | Wolters Kluwer |
Rights: | Aberto |
Identifier DOI: | 10.1016/j.apjtb.2016.07.007 |
Address: | https://www.sciencedirect.com/science/article/pii/S222116911630096X |
Date Issue: | 2016 |
Appears in Collections: | FEA - Artigos e Outros Documentos CPQBA - Artigos e Outros Documentos FCF - Artigos e Outros Documentos |
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