Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/351323
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampCosta, Gustavo Gilson Lacerda-
dc.contributor.authorunicampThomazella, Daniela Paula de Toledo-
dc.contributor.authorunicampTeixeira, Paulo José Pereira Lima-
dc.contributor.authorunicampPereira, Gonçalo Amarante Guimarães-
dc.contributor.authorunicampCarazzolle, Marcelo Falsarella-
dc.contributor.authorunicampCosta, Gustavo Gilson Lacerda-
dc.typeArtigopt_BR
dc.titleGenome and secretome analysis of the hemibiotrophic fungal pathogen, Moniliophthora roreri, which causes frosty pod rot disease of cacao: mechanisms of the biotrophic and necrotrophic phasespt_BR
dc.contributor.authorMeinhardt, Lyndel W.-
dc.contributor.authorCosta, Gustavo Gilson Lacerda-
dc.contributor.authorThomazella, Daniela P. T.-
dc.contributor.authorTeixeira, Paulo José P. L.-
dc.contributor.authorCarazzolle, Marcelo Falsarella-
dc.contributor.authorSchuster, Stephan C.-
dc.contributor.authorCarlson, John E.-
dc.contributor.authorGuiltinan, Mark J.-
dc.contributor.authorMieczkowski, Piotr-
dc.contributor.authorFarmer, Andrew-
dc.contributor.authorRamaraj, Thiruvarangan-
dc.contributor.authorCrozier, Jayne-
dc.contributor.authorDavis, Robert E.-
dc.contributor.authorShao, Jonathan-
dc.contributor.authorMelnick, Rachel L.-
dc.contributor.authorPereira, Gonçalo A. G.-
dc.contributor.authorBailey, Bryan A.-
dc.subjectMoniliophthora roreript_BR
dc.subject.otherlanguageMoniliophthora roreript_BR
dc.description.abstractBackground: The basidiomycete Moniliophthora roreri is the causal agent of Frosty pod rot (FPR) disease of cacao (Theobroma cacao), the source of chocolate, and FPR is one of the most destructive diseases of this important perennial crop in the Americas. This hemibiotroph infects only cacao pods and has an extended biotrophic phase lasting up to sixty days, culminating in plant necrosis and sporulation of the fungus without the formation of a basidiocarp.Results: We sequenced and assembled 52.3 Mb into 3,298 contigs that represent the M. roreri genome. Of the 17,920 predicted open reading frames (OFRs), 13,760 were validated by RNA-Seq. Using read count data from RNA sequencing of cacao pods at 30 and 60 days post infection, differential gene expression was estimated for the biotrophic and necrotrophic phases of this plant-pathogen interaction. The sequencing data were used to develop a genome based secretome for the infected pods. Of the 1,535 genes encoding putative secreted proteins, 1,355 were expressed in the biotrophic and necrotrophic phases. Analysis of the data revealed secretome gene expression that correlated with infection and intercellular growth in the biotrophic phase and invasive growth and plant cellular death in the necrotrophic phase.Conclusions: Genome sequencing and RNA-Seq was used to determine and validate the Moniliophthora roreri genome and secretome. High sequence identity between Moniliophthora roreri genes and Moniliophthora perniciosa genes supports the taxonomic relationship with Moniliophthora perniciosa and the relatedness of this fungus to other basidiomycetes. Analysis of RNA-Seq data from infected plant tissues revealed differentially expressed genes in the biotrophic and necrotrophic phases. The secreted protein genes that were upregulated in the biotrophic phase are primarily associated with breakdown of the intercellular matrix and modification of the fungal mycelia, possibly to mask the fungus from plant defenses. Based on the transcriptome data, the upregulated secreted proteins in the necrotrophic phase are hypothesized to be actively attacking the plant cell walls and plant cellular components resulting in necrosis. These genes are being used to develop a new understanding of how this disease interaction progresses and to identify potential targets to reduce the impact of this devastating diseasept_BR
dc.relation.ispartofBMC genomicspt_BR
dc.publisher.cityLondonpt_BR
dc.publisher.countryReino Unidopt_BR
dc.publisherSpringer Naturept_BR
dc.date.issued2014-
dc.date.monthofcirculationFeb.pt_BR
dc.language.isoengpt_BR
dc.description.volume15pt_BR
dc.description.issuenumber1pt_BR
dc.rightsFechadopt_BR
dc.sourceSCOPUSpt_BR
dc.identifier.eissn1471-2164pt_BR
dc.identifier.doi10.1186/1471-2164-15-164pt_BR
dc.identifier.urlhttps://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-15-164pt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumber2013/08293-7pt_BR
dc.date.available2020-10-20T11:02:54Z-
dc.date.accessioned2020-10-20T11:02:54Z-
dc.description.provenanceSubmitted by Sanches Olivia (olivias@unicamp.br) on 2020-10-20T11:02:54Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-02-11T21:11:57Z : No. of bitstreams: 1 2-s2.0-84896719858.pdf: 2318560 bytes, checksum: 2447f36cd2755c801ab9cb1d539b709d (MD5)en
dc.description.provenanceMade available in DSpace on 2020-10-20T11:02:54Z (GMT). No. of bitstreams: 0 Previous issue date: 2014en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/351323-
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentDepartamento de Genética, Evolução, Microbiologia e Imunologiapt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.identifier.source2-s2.0-84896719858pt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcid0000-0003-2041-8714pt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcid0000-0002-5474-2830pt_BR
dc.creator.orcid0000-0003-4140-3482pt_BR
dc.type.formArtigo de pesquisapt_BR
dc.identifier.articleid164pt_BR
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