Please use this identifier to cite or link to this item:
Type: Artigo
Title: New hydrocarbon degradation pathways in the microbial metagenome from brazilian petroleum reservoirs
Author: Alvarez, J. C.
de Oliveira, V. M.
Sierra-Garcia, I. N.
dos Santos, E. V.
de Souza, A. P.
de Vasconcellos, S. P.
Abstract: Current knowledge of the microbial diversity and metabolic pathways involved in hydrocarbon degradation in petroleum reservoirs is still limited, mostly due to the difficulty in recovering the complex community from such an extreme environment. Metagenomics is a valuable tool to investigate the genetic and functional diversity of previously uncultured microorganisms in natural environments. Using a function-driven metagenomic approach, we investigated the metabolic abilities of microbial communities in oil reservoirs. Here, we describe novel functional metabolic pathways involved in the biodegradation of aromatic compounds in a metagenomic library obtained from an oil reservoir. Although many of the deduced proteins shared homology with known enzymes of different well-described aerobic and anaerobic catabolic pathways, the metagenomic fragments did not contain the complete clusters known to be involved in hydrocarbon degradation. Instead, the metagenomic fragments comprised genes belonging to different pathways, showing novel gene arrangements. These results reinforce the potential of the metagenomic approach for the identification and elucidation of new genes and pathways in poorly studied environments and contribute to a broader perspective on the hydrocarbon degradation processes in petroleum reservoirs
Subject: Biodegradação
Country: Estados Unidos
Editor: Public Library of Science
Rights: Aberto
Identifier DOI: 10.1371/journal.pone.0090087
Date Issue: 2014
Appears in Collections:IB - Artigos e Outros Documentos
IB - Artigos e Outros Documentos
CBMEG - Artigos e Outros Documentos

Files in This Item:
File Description SizeFormat 
000332389000133.pdf2.27 MBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.