Lipase selective entrapment in alginate from Burkholderia cepacia crude extract

Abstract

Considered one of the most important enzymes for the biotechnology industries, lipase is a very versatile biocatalyst been target of several researches and review papers and used in many different industrial applications, such as household detergent additive, chemicals and pharmaceutical compounds synthesis, biodiesel production and others. Lipase produced by Burkholderia cepacia was entrapped by additon of the crude extract and alginate solution in Ca++ hardening solution. Using factorial design of experiments, the procedure conditions were studied: alginate solution concentration, Ca++ concentration, ph of solution, bead size and crude extract/alginate solution proportions. In a second step of the work, the alginate solution concentration, calcium concentration and Ph were evaluated against responses relating to entrapment of the lipase and purification of the biocatalyst using a surface responde methodology. In a single-step procedure it was possible to entrap lipase directly from crude achievig purification factors of 1.69 (168.07 % immobilization yield) with activity recoveries around 99%.