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Type: Artigo
Title: Expanded bed adsorption of an alkaline lipase from Pseudomona cepacia
Author: Padilha, Giovana da Silva
Curvelo-Santana, José Carlos
Alegre, Ranulfo Monte
Tambourgi, Elias Basile
Abstract: An extracellular lipase was isolated from Pseudomona cepacia by expanded bed adsorption on an Amberlite 410 ion-exchange resin. Enzyme characterization and hydrodynamic study of a chromatography column were done. Enzyme purification was done at three condition of expanded bed height (H): at one and half (6 cm), at two (8 cm) and at three (12 cm) times the fixed bed height (H0 = 4 cm). The results showed that the experimental data was fitted to the Richardson and Zaki equation, and the comparison between the experimental and calculated terminal velocities showed low relative error. In enzyme purification for better condition, a purification factor of about 80 times was found at 6 cm of expanded bed height, or 1.5 times of expansion degree. Purified lipase had an optimal pH and a temperature of 8 and 37 °C, respectively.
Subject: Adsorção
Country: Países Baixos
Editor: Elsevier
Rights: Fechado
Identifier DOI: 10.1016/j.jchromb.2008.12.061
Date Issue: 2009
Appears in Collections:FEA - Artigos e Outros Documentos

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