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Type: Artigo
Title: Influence of culture conditions on glutathione production by Saccharomyces cerevisiae
Author: Santos, Lucielen Oliveira
Gonzales, Tatiane Araujo
Úbeda, Beatriz Torsani
Alegre, Ranulfo Monte
Abstract: A strategy of experimental design using a fractional factorial design (FFD) and a central composite rotatable design (CCRD) were carried out with the aim to obtain the best conditions of temperature (20–30°C), agitation rate (100–300 rpm), initial pH (5.0–7.0), inoculum concentration (5–15%), and glucose concentration (30–70 g/l) for glutathione (GSH) production in shake-flask culture by Saccharomyces cerevisiae ATCC 7754. By a FFD (25–2), the agitation rate, temperature, and pH were found to be significant factors for GSH production. In CCRD (22) was obtained a second-order model equation, and the percent of variation explained by the model was 95%. The results showed that the optimal culture conditions were agitation rate, 300 rpm; temperature, 20°C; initial pH, 5; glucose, 54 g/l; and inoculum concentration, 5%. The highest GSH concentration (154.5 mg/l) was obtained after 72 h of fermentation.
Subject: Saccharomyces cerevisiae
Country: Alemanha
Editor: Springer
Rights: Fechado
Identifier DOI: 10.1007/s00253-007-1211-6
Date Issue: 2007
Appears in Collections:FEA - Artigos e Outros Documentos

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