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|Title:||Alginate production by Pseudomonas mendocina in a stirred draft fermenter|
|Author:||Müller, José Miguel|
Alegre, Ranulfo Monte
|Abstract:||In this study alginate production by Pseudomonas mendocina in a laboratory-scale fermenter was investigated. In the experiments the effect of temperature (25–31°C) and agitation (500–620 rev min−1) at a constant air flow of 10 v/v/h were evaluated in relation to the rate of glucose bioconversion to alginate using response surface methodology (RSM). The fermenter configuration was also adapted to a system with a screw mixer and draft tube, due to the change in rheological characteristics of the fermentation broth. The adjusted model indicates a temperature of 29.1°C and agitation of 553 rev min−1 for optimum alginate synthesis. In this fermentation system a Y p/s of 44.8% was achieved. The alginate synthesized by P. mendocina showed a partially acetylated pattern as previously reported for alginates obtained from other Pseudomonas spp and Azotobacter vinelandii.|
|Appears in Collections:||FEA - Artigos e Outros Documentos|
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