Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/351023
Type: Artigo
Title: Enzyme prodrug therapy engineered into electrospun fibers with embedded liposomes for controlled, localized synthesis of therapeutics
Author: Chandrawati, Rona
Olesen, Morten T. J.
Marini, Thatiane C. C.
Bisra, Gurpal
Guex, Anne Géraldine
Oliveira, Marcelo G. de
Zelikin, Alexander N.
Stevens, Molly M.
Abstract: Enzyme prodrug therapy (EPT) enables localized conversion of inert prodrugs to active drugs by enzymes. Performance of EPT necessitates that the enzyme remains active throughout the time frame of the envisioned therapeutic application. β‐glucuronidase is an enzyme with historically validated performance in EPT, however it retains its activity in biomaterials for an insufficiently long period of time, typically not exceeding 7 d. Herein, the encapsulation of β‐glucuronidase in liposomal subcompartments within poly(vinyl alcohol) electrospun fibers is reported, leading to the assembly of biocatalytically active materials with activity of the enzyme sustained over at least seven weeks. It is further shown that liposomes provide the highly beneficial stabilization of the enzyme when incubated in cell culture media. The assembled biocatalytic materials successfully produce antiproliferative drugs (SN‐38) using externally administered prodrugs (SN‐38‐glucuronide) and effectively suppress cell proliferation, with envisioned utility in the design of cardiovascular grafts
Subject: Fibras
Lipossomas
Eletrofiação
Country: Alemanha
Editor: John Wiley & Sons
Rights: Fechado
Identifier DOI: 10.1002/adhm.201700385
Address: https://onlinelibrary.wiley.com/doi/full/10.1002/adhm.201700385
Date Issue: 2017
Appears in Collections:IQ - Artigos e Outros Documentos

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