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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampRosa, Renata Giardini-
dc.contributor.authorunicampJoazeiro, Paulo Pinto-
dc.typeArtigopt_BR
dc.titleDevelopment of scaffold-free elastic cartilaginous constructs with structural similarities to auricular cartilagept_BR
dc.contributor.authorGiardini-Rosa, R.-
dc.contributor.authorThomas, K.-
dc.contributor.authorWaldman, S. D.-
dc.contributor.authorWeber, J.-
dc.contributor.authorJoazeiro, P. P.-
dc.contributor.authorCollavino, K.-
dc.subjectSínfise púbicapt_BR
dc.subjectCartilagem articularpt_BR
dc.subjectCélulas-troncopt_BR
dc.subjectCondrócitospt_BR
dc.subject.otherlanguagePubic symphysispt_BR
dc.subject.otherlanguageArticular cartilagept_BR
dc.subject.otherlanguageStem cellspt_BR
dc.subject.otherlanguageChondrocytespt_BR
dc.description.abstractExternal ear reconstruction with autologous cartilage still remains one of the most difficult problems in the fields of plastic and reconstructive surgery. As the absence of tissue vascularization limits the ability to stimulate new tissue growth, relatively few surgical approaches are currently available (alloplastic implants or sculpted autologous cartilage grafts) to repair or reconstruct the auricle (or pinna) as a result of traumatic loss or congenital absence (e.g., microtia). Alternatively, tissue engineering can offer the potential to grow autogenous cartilage suitable for implantation. While tissue-engineered auricle cartilage constructs can be created, a substantial number of cells are required to generate sufficient quantities of tissue for reconstruction. Similarly, as routine cell expansion can elicit negative effects on chondrocyte function, we have developed an approach to generate large-sized engineered auricle constructs (>= 3 cm(2)) directly from a small population of donor cells (20,000-40,000 cells/construct). Using rabbit donor cells, the developed bioreactor-cultivated constructs adopted structural-like characteristics similar to native auricular cartilage, including the development of distinct cartilaginous and perichondrium-like regions. Both alterations in media composition and seeding density had profound effects on the formation of engineered elastic tissue constructs in terms of cellularity, extracellular matrix accumulation, and tissue structure. Higher seeding densities and media containing sodium bicarbonate produced tissue constructs that were closer to the native tissue in terms of structure and composition. Future studies will be aimed at improving the accumulation of specific tissue constituents and determining the clinical effectiveness of this approach using a reconstructive animal modelpt_BR
dc.relation.ispartofTissue engineering. Part Apt_BR
dc.relation.ispartofabbreviationTissue eng. Part Apt_BR
dc.publisher.cityNew Rochelle, NYpt_BR
dc.publisher.countryEstados Unidospt_BR
dc.publisherMary Ann Liebertpt_BR
dc.date.issued2014-
dc.date.monthofcirculationMar.pt_BR
dc.language.isoengpt_BR
dc.description.volume20pt_BR
dc.description.issuenumber5-6pt_BR
dc.description.firstpage1012pt_BR
dc.description.lastpage1026pt_BR
dc.rightsFechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn1937-3341pt_BR
dc.identifier.eissn1937-335Xpt_BR
dc.identifier.doi10.1089/ten.tea.2013.0159pt_BR
dc.identifier.urlhttps://www.liebertpub.com/doi/10.1089/ten.tea.2013.0159pt_BR
dc.date.available2020-10-14T15:21:48Z-
dc.date.accessioned2020-10-14T15:21:48Z-
dc.description.provenanceSubmitted by Cintia Oliveira de Moura (cintiaom@unicamp.br) on 2020-10-14T15:21:47Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-02-16T16:52:26Z : No. of bitstreams: 1 000332021600013.pdf: 1041222 bytes, checksum: 4b83803e1212eb036fc142c2f0cbf1a9 (MD5)en
dc.description.provenanceMade available in DSpace on 2020-10-14T15:21:48Z (GMT). No. of bitstreams: 0 Previous issue date: 2014en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/351016-
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentDepartamento de Bioquímica e Biologia Tecidualpt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.identifier.source000332021600013pt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcid0000-0003-4726-6159pt_BR
dc.type.formArtigo originalpt_BR
dc.description.sponsorNoteCanadian Institutes of Health Research (CIHR); Foreign Affairs; International Trade Canada/L'appui d'Affaires etrangeres et Commerce international Canadapt_BR
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