Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/350984
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampCarazzolle, Marcelo Falsarella-
dc.contributor.authorunicampPereira, Gonçalo Amarante Guimarães-
dc.typeArtigopt_BR
dc.titleAnalysis of the ergosterol biosynthesis pathway cloning, molecular characterization and phylogeny of lanosterol 14α-demethylase (ERG11) gene of Moniliophthora perniciosapt_BR
dc.contributor.authorCeita, Geruza de Oliveira-
dc.contributor.authorVilas-Boas, Laurival Antônio-
dc.contributor.authorCastilho, Marcelo Santos-
dc.contributor.authorCarazzolle, Marcelo Falsarella-
dc.contributor.authorPirovani, Carlos Priminho-
dc.contributor.authorSelbach-Schnadelbach, Alessandra-
dc.contributor.authorGramacho, Karina Peres-
dc.contributor.authorRamos, Pablo Ivan Pereira-
dc.contributor.authorBarbosa, Luciana Veiga-
dc.contributor.authorPereira, Gonçalo Amarante Guimarães-
dc.contributor.authorGóes-Neto, Aristóteles-
dc.subjectCacaueiropt_BR
dc.subject.otherlanguageCacaopt_BR
dc.description.abstractThe phytopathogenic fungus Moniliophthora perniciosa (Stahel) Aime & Philips-Mora, causal agent of witches' broom disease of cocoa, causes countless damage to cocoa production in Brazil. Molecular studies have attempted to identify genes that play important roles in fungal survival and virulence. In this study, sequences deposited in the M. perniciosa Genome Sequencing Project database were analyzed to identify potential biological targets. For the first time, the ergosterol biosynthetic pathway in M. perniciosa was studied and the lanosterol 14α-demethylase gene (ERG11) that encodes the main enzyme of this pathway and is a target for fungicides was cloned, characterized molecularly and its phylogeny analyzed. ERG11 genomic DNA and cDNA were characterized and sequence analysis of the ERG11 protein identified highly conserved domains typical of this enzyme, such as SRS1, SRS4, EXXR and the heme-binding region (HBR). Comparison of the protein sequences and phylogenetic analysis revealed that the M. perniciosa enzyme was most closely related to that of Coprinopsis cinereapt_BR
dc.relation.ispartofGenetics and molecular biologypt_BR
dc.relation.ispartofabbreviationGenet. mol. biol.pt_BR
dc.publisher.cityRibeirão Preto, SPpt_BR
dc.publisher.countryBrasilpt_BR
dc.publisherSociedade Brasileira de Genéticapt_BR
dc.date.issued2014-
dc.date.monthofcirculationOct.pt_BR
dc.language.isoengpt_BR
dc.description.volume37pt_BR
dc.description.issuenumber4pt_BR
dc.description.firstpage683pt_BR
dc.description.lastpage693pt_BR
dc.rightsAbertopt_BR
dc.sourceSCOPUSpt_BR
dc.identifier.issn1415-4757pt_BR
dc.identifier.eissn1678-4685pt_BR
dc.identifier.doi10.1590/S1415-47572014005000017pt_BR
dc.identifier.urlhttps://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500011pt_BR
dc.description.sponsorshipFINANCIADORA DE ESTUDOS E PROJETOS - FINEPpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DA BAHIA - FAPESBpt_BR
dc.description.sponsordocumentnumbersem informaçãopt_BR
dc.date.available2020-10-13T23:50:48Z-
dc.date.accessioned2020-10-13T23:50:48Z-
dc.description.provenanceSubmitted by Sanches Olivia (olivias@unicamp.br) on 2020-10-13T23:50:48Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-02-11T21:15:01Z : No. of bitstreams: 1 2-s2.0-84914703975.pdf: 2316490 bytes, checksum: f68ae93083e71c5892d8d4bf9b9378ce (MD5)en
dc.description.provenanceMade available in DSpace on 2020-10-13T23:50:48Z (GMT). No. of bitstreams: 0 Previous issue date: 2014en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/350984-
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentDepartamento de Genética, Evolução e Bioagentespt_BR
dc.contributor.departmentDepartamento de Genética, Evolução, Microbiologia e Imunologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.subject.keywordBasidiomycotapt_BR
dc.subject.keywordFunguspt_BR
dc.subject.keywordErgosterolpt_BR
dc.identifier.source2-s2.0-84914703975pt_BR
dc.creator.orcid0000-0002-5474-2830pt_BR
dc.creator.orcid0000-0003-4140-3482pt_BR
dc.type.formArtigo de pesquisapt_BR
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