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|Title:||Effects of pamidronate on dental enamel formation assessed by light microscopy, energy-dispersive X-Ray analysis, scanning electron microscopy, and microhardness testing|
|Author:||Soares, Ana P.|
do Espírito Santo, Renan F.
Line, Sérgio R. P.
Pinto, Maria das G. F.
Santos, Pablo de M.
Toralles, Maria Betania P.
do Espírito Santo, Alexandre R.
|Abstract:||The aim of the present work was to investigate birefringence and morphology of the secretory-stage enamel organic extracellular matrix (EOECM), and structural and mechanical properties of mature enamel of upper incisors from adult rats that had been treated with pamidronate disodium (0.5 mg/kg/week for 56 days), using transmitted polarizing and bright-field light microscopies (TPLM and BFLM), energy-dispersive X-ray (EDX) analysis, scanning electron microscopy (SEM) and microhardness testing. BFLM showed no morphological changes of the EOECM in pamidronate and control groups, but TPLM revealed a statistically significant reduction in optical retardation values of birefringence brightness of pamidronate-treated rats when compared with control animals (p<0.01). EDX analysis showed that pamidronate-induced statistically significant decrease in phosphorus' quantity in outer mature enamel (p<0.01) and an increase in the calcium/phosphorus ratio in that structure (p<0.05). Pamidronate did not induce ultra-structural alterations in mature enamel as revealed by SEM and did not cause a reduction in its microhardness (p>0.05). The present study indicates that pamidronate can affect birefringence of the secretory-stage EOECM, which does not seem to be associated with significant changes in morphological and/or mechanical properties of mature enamel|
|Subject:||Microscopia eletrônica de varredura|
|Editor:||Cambridge University Press|
|Appears in Collections:||FOP - Artigos e Outros Documentos|
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