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Type: | Artigo |
Title: | Comparative study of the bioconversion process using R-(+)- and S-(–)-limonene as substrates for fusarium oxysporum 152B |
Author: | Molina, Gustavo Bution, Murillo L. Bicas, Juliano L. Dolder, Mary Anne Heidi Pastore, Gláucia M. |
Abstract: | This study compared the bioconversion process of S-(–)-limonene into limonene-1,2-diol with the already established biotransformation of R-(+)-limonene into α-terpineol using the same biocatalyst in both processes, Fusarium oxysporum 152B. The bioconversion of the S-(−)-isomer was tested on cell permeabilisation under anaerobic conditions and using a biphasic system. When submitted to permeabilisation trials, this biocatalyst has shown a relatively high resistance; still, no production of limonene-1,2-diol and a loss of activity of the biocatalyst were observed after intense cell treatment, indicating a complete loss of cell viability. Furthermore, the results showed that this process can be characterised as an aerobic system that was catalysed by limonene-1,2-epoxide hydrolase, had an intracellular nature and was cofactor-dependent because the final product was not detected by an anaerobic process. Finally, this is the first report to characterise the bioconversion of R-(+)- and S-(–)-limonene by cellular detoxification using ultra-structural analysis |
Subject: | Monoterpenos |
Country: | Países Baixos |
Editor: | Elsevier |
Rights: | Fechado |
Identifier DOI: | 10.1016/j.foodchem.2014.11.059 |
Address: | https://www.sciencedirect.com/science/article/pii/S0308814614017889 |
Date Issue: | 2015 |
Appears in Collections: | IB - Artigos e Outros Documentos FEA - Artigos e Outros Documentos |
Files in This Item:
File | Description | Size | Format | |
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000348088000083.pdf | 2.19 MB | Adobe PDF | View/Open |
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