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|Title:||Improving bioethanol production by scheffersomyces stipitis using retentostat extractive fermentation at high xylose concentration|
Atala, Daniel Ibraim Pires
|Abstract:||The improvement of yields and productivity for second-generation ethanol (2G) production as well as a better understanding of industrial yeasts and processes are required. Nevertheless, a process to produce 2G ethanol efficiently from lignocellulosic C5-sugars has not yet been proposed. Scheffersomyces stipitis presents good fermentation performance, promising to be a potential biocatalyst for converting C5-sugars to ethanol. In order to evaluate yeast performance, experiments were carried out in a retentostatic extractive vacuum system (REV) with total cell retention, controlled substrate feeding (at different xylose concentrations 100–200 g L−1) and simultaneous ethanol production and extraction. During REV at 100 g L−1, it was possible to achieve an efficient ethanol production. The optimum dilution rate was 0.025 h−1. To avoid stress effects to the yeast metabolism, the ethanol was continuously extracted. Low ethanol concentrations were kept inside the fermenter (25–35 g L‐1), resulting in higher yields and productivity when compared with batch, fed-batch and continuous cultures|
|Appears in Collections:||FEA - Artigos e Outros Documentos|
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