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Type: Artigo
Title: Enzymatic biodiesel synthesis from yeast oil using immobilized recombinant rhizopus oryzae lipase
Author: Duarte, Susan Hartwig
Hernández, Gonzalo Lázaro del Peso
Canet, Albert
Benaiges, Maria Dolors
Maugeri, Francisco
Valero, Francisco
Abstract: The recombinant Rhizopus oryzae lipase (1–3 positional selective), immobilized on Relizyme OD403, has been applied to the production of biodiesel using single cell oil from Candida sp. LEB-M3 growing on glycerol from biodiesel process. The composition of microbial oil is quite similar in terms of saponifiable lipids than olive oil, although with a higher amount of saturated fatty acids. The reaction was carried out in a solvent system, and n-hexane showed the best performance in terms of yield and easy recovery. The strategy selected for acyl acceptor addition was a stepwise methanol addition using crude and neutralized single cell oil, olive oil and oleic acid as substrates. A FAMEs yield of 40.6% was obtained with microbial oils lower than olive oil 54.3%. Finally in terms of stability, only a lost about 30% after 6 reutilizations were achieved
Subject: Biodiesel
Country: Países Baixos
Editor: Elsevier
Rights: Fechado
Identifier DOI: 10.1016/j.biortech.2015.01.133
Date Issue: 2015
Appears in Collections:FEA - Artigos e Outros Documentos

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