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Type: Artigo
Title: Validation of the sulfo-phospho-vanillin (SPV) method for the determination of lipid content in oleaginous microorganisms
Author: Anschau, A.
Caruso, C. S.
Kuhn, R. C.
Franco, T. T.
Abstract: A colorimetric sulfo-phospho-vanillin (SPV) method was used to validate a high throughput method for total lipids analysis in fresh and lyophilized oleaginous microorganisms. This method uses a small amount of biological material, does not require a lot of sample manipulation, can be automated, is reproducible and easy to implement. A 96-well microplate SPV assay allows one to determine quickly total lipids in lyophilized cells of oleaginous yeast and microalgae. The new assay method possesses many advantages compared to the others described in the literature: requires a small amount sample, less time (around 1 h) and less labor and does not require organic reagents like chloroform in the reaction
Subject: Lipídeos
Country: Brasil
Editor: Associação Brasileira de Engenharia Química
Rights: Aberto
Identifier DOI: 10.1590/0104-6632.20170341s20140222
Date Issue: 2017
Appears in Collections:FEQ - Artigos e Outros Documentos

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