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Type: Artigo
Title: Production of cyclodextrin glycosyltransferase by immobilized bacillus sp. on chitosan matrix
Author: Eş, Ismail
Ribeiro, Maycon Carvalho
Santos Júnior, Samuel Rodrigues dos
Khaneghah, Amin Mousavi
Rodriguez, Armando Garcia
Amaral, André Corrêa
Abstract: The whole-cell immobilization on chitosan matrix was evaluated. Bacillus sp., as producer of CGTase, was grown in solid-state and batch cultivation using three types of starches (cassava, potato and cornstarch). Biomass growth and substrate consumption were assessed by flow cytometry and modified phenol–sulfuric acid assays, respectively. Qualitative analysis of CGTase production was determined by colorless area formation on solid culture containing phenolphthalein. Scanning electron microscopy (SEM) analysis demonstrated that bacterial cells were immobilized on chitosan matrix efficiently. Free cells reached very high numbers during batch culture while immobilized cells maintained initial inoculum concentration. The maximum enzyme activity achieved by free cells was 58.15 U ml−1 (36 h), 47.50 U ml−1 (36 h) and 68.36 U ml−1 (36 h) on cassava, potato and cornstarch, respectively. CGTase activities for immobilized cells were 82.15 U ml−1 (18 h) on cassava, 79.17 U ml−1 (12 h) on potato and 55.37 U ml−1 (in 6 h and max 77.75 U ml−1 in 36 h) on cornstarch. Application of immobilization technique increased CGTase activity significantly. The immobilized cells produced CGTase with higher activity in a shorter fermentation time comparing to free cells
Subject: Ciclodextrinas
Country: Alemanha
Editor: Springer
Rights: Fechado
Identifier DOI: 10.1007/s00449-016-1625-6
Date Issue: 2016
Appears in Collections:FEA - Artigos e Outros Documentos

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