Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/350070
Type: TESE DIGITAL
Degree Level: Doutorado
Title: Bovine mastitis : natural products as alternatives for treatment =Mastite bovina : produtos naturais como alternativas de tratamento
Title Alternative: Mastite bovina : produtos naturais como alternativas de tratamento
Author: Martins, Thamires, 1987-
Advisor: Reyes, Felix Guillermo Reyes, 1948-
Abstract: Resumo: A mastite bovina é uma patologia frequente e que mais causa prejuízos econômicos à indústria leiteira, sendo o uso de antimicrobianos a principal ferramenta para o tratamento. O insucesso dos tratamentos com antimicrobianos existentes diante de bactérias multirresistentes está cada vez mais comum o que torna urgente a busca por novos fármacos eficazes contra os patógenos da mastite. Os microrganismos e as plantas são fontes promissoras de produtos naturais com ação antibacteriana. Assim, o objetivo desta tese foi buscar produtos naturais com atividade antibacteriana e avaliar o potencial para serem utilizados no tratamento da mastite bovina. No período de 2010-2015, mais de 50 moléculas antibacterianas produzidas por bactérias obtidas de substratos marinhos foram relatadas na literatura. Entre elas, anthracimycin, kocurin, macrolactins A1 e B1, gageotetrins A?C e gageomacrolactins 1-3 são exemplos de moléculas com atividade antibacteriana promissora e que podem ser investigadas para o desenvolvimento de novos medicamentos. Localizado no centro do oceano Atlântico, o Arquipélago São Pedro e São Paulo (ASPSP) é um local com características únicas e que pode oferecer uma ampla biodiversidade pouco estudada. Microrganismos isolados de esponjas marinhas coletadas nas proximidades do ASPSP produziram metabólitos secundários com atividade potencial contra Staphylococcus aureus resistentes à meticilina (MRSA). Um destes foi o fungo Acremonium camptosporum, um microrganismo ubíquo, isolado pela primeira vez da esponja Aplysina fulva. A análise por espectrometria de massas (UPLC-ESI-MS/MS) do extrato deste fungo revelou a presença de diversas acremonidins e acremoxantones. Esses compostos são conhecidos por apresentarem alta citotoxicidade, portanto, não foram considerados bons candidatos para serem utilizados em formulações veterinárias. No entanto, verificou-se que o arsenal químico produzido pelo A. camptosporum pode ter um papel fundamental tanto na defesa da esponja quanto do próprio fungo. Por outro lado, os óleos essenciais são produtos naturais conhecidos por suas diversificadas propriedades biológicas. Assim, o óleo essencial (OE) de Baccharis dracunculifolia foi submetido a um fracionamento por flash cromatografia, tendo-se obtido frações contendo alto teor de seus compostos majoritários, trans-nerolidol (91,47% ¿ 96,14%) e spathulenol (78,63 ¿ 84,89%). O OE e as frações trans-nerolidol e spathulenol inibiram o crescimento de linhagens de S. aureus (MIC90= 375 µg mL-1) e Streptococcus agalactiae (MIC90= 187.5 µg mL-1) isolados do leite de animais com mastite bovina. Contudo, nas concentrações mínimas inibitórias dos patógenos, essas amostras mostraram redução de mais de 50% da viabilidade das células epiteliais mamárias de bovinos (MAC-T) e de queratinócitos (HaCaT). Portanto, não são recomendados para serem utilizados em formulações para o tratamento de mastite nessas concentrações. No entanto, os compostos trans-nerolidol e spathulenol apresentam outras propriedades farmacológicas relatadas na literatura e que podem ser investigadas com a finalidade de prevenir e/ou auxiliar no tratamento da mastite bovina

Abstract: Bovine mastitis is a frequent pathology that causes high economic negative impact to the dairy industry. Antimicrobials are the main tool to treat this disease. The failure of existing antimicrobial treatments against multidrug resistant bacteria is increasingly common, making the search for new drugs effective against mastitis pathogens more urgent. Microorganisms and plants are promising sources of natural products with antibacterial action. Thus, the aim of this thesis was to search for natural products with antibacterial activity and to evaluate their potential use in the bovine mastitis treatment. In the period of 2010-2015, there were reported in the literature more than 50 antibacterial molecules obtained from marine substrates. Among them, anthracimycin, kocurin, macrolactins A1 and B1, gageotetrins A-C and gageomacrolactins 1-3 are examples of molecules with promising antibacterial activity and that could be investigated for the development of new drugs. Located at mid-Atlantic Ocean, the São Pedro and São Paulo Archipelago (ASPSP) is a place with unique characteristics that could offer a wide biodiversity, nevertheless it has been little studied. Microorganisms isolated from marine sponges collected around the ASPSP produced secondary metabolites with potential activity against methicillin-resistant Staphylococcus aureus (MRSA). One of them was the fungus Acremonium camptosporum, a ubiquitous microorganism isolated for the first time from the sponge Aplysina fulva. Mass spectra data obtained by UPLC-ESI-MS/MS revealed the presence of several acremonidins and acremoxanthones in the mycelial extract. These compounds are known to present high cytotoxicity, therefore, they were not considered as candidates to be used in veterinary formulations. However, it was verified that the chemical arsenal produced by A. camptosporum might play a fundamental role in the defense of the sponge, as well as of the fungus itself. On the other hand, essential oils are natural products known for their diverse biological properties. Thus, the essential oil (OE) from Baccharis dracunculifolia was subjected to a flash chromatography fractionation, obtaining fractions with high content of its major compounds, trans-nerolidol (91.47 - 96.14%) and spathulenol (78.63 - 84.89%). The OE and the trans-nerolidol and spathulenol fractions inhibited the growth of several S. aureus (MIC90 = 375 µg mL-1) and Streptococcus agalactiae (MIC90 = 187.5 Bovine mastitis is a frequent pathology that causes high economic negative impact to the dairy industry. Antimicrobials are the main tool to treat this disease. The failure of existing antimicrobial treatments against multidrug resistant bacteria is increasingly common, making the search for new drugs effective against mastitis pathogens more urgent. Microorganisms and plants are promising sources of natural products with antibacterial action. Thus, the aim of this thesis was to search for natural products with antibacterial activity and to evaluate their potential use in the bovine mastitis treatment. In the period of 2010-2015, there were reported in the literature more than 50 antibacterial molecules obtained from marine substrates. Among them, anthracimycin, kocurin, macrolactins A1 and B1, gageotetrins A-C and gageomacrolactins 1-3 are examples of molecules with promising antibacterial activity and that could be investigated for the development of new drugs. Located at mid-Atlantic Ocean, the São Pedro and São Paulo Archipelago (ASPSP) is a place with unique characteristics that could offer a wide biodiversity, nevertheless it has been little studied. Microorganisms isolated from marine sponges collected around the ASPSP produced secondary metabolites with potential activity against methicillin-resistant Staphylococcus aureus (MRSA). One of them was the fungus Acremonium camptosporum, a ubiquitous microorganism isolated for the first time from the sponge Aplysina fulva. Mass spectra data obtained by UPLC-ESI-MS/MS revealed the presence of several acremonidins and acremoxanthones in the mycelial extract. These compounds are known to present high cytotoxicity, therefore, they were not considered as candidates to be used in veterinary formulations. However, it was verified that the chemical arsenal produced by A. camptosporum might play a fundamental role in the defense of the sponge, as well as of the fungus itself. On the other hand, essential oils are natural products known for their diverse biological properties. Thus, the essential oil (OE) from Baccharis dracunculifolia was subjected to a flash chromatography fractionation, obtaining fractions with high content of its major compounds, trans-nerolidol (91.47 - 96.14%) and spathulenol (78.63 - 84.89%). The OE and the trans-nerolidol and spathulenol fractions inhibited the growth of several S. aureus (MIC90 = 375 µg mL-1) and Streptococcus agalactiae (MIC90 = 187.5 µg mL-1) isolated from milk of animals with bovine mastitis. However, at the minimal inhibitory concentrations, these samples showed a decrease of more than 50% of bovine mammary epithelial cells (MAC-T) and keratinocytes (HaCaT) viability. Therefore, they are not recommended to be used at such concentrations in formulations intended for mastitis treatment purposes. Nevertheless, other pharmacological properties of trans-nerolidol and spathulenol were previously reported and could be investigated for preventing purposes and/or as adjuvant in the bovine mastitis treatment. g mL-1) isolated from milk of animals with bovine mastitis. However, at the minimal inhibitory concentrations, these samples showed a decrease of more than 50% of bovine mammary epithelial cells (MAC-T) and keratinocytes (HaCaT) viability. Therefore, they are not recommended to be used at such concentrations in formulations intended for mastitis treatment purposes. Nevertheless, other pharmacological properties of trans-nerolidol and spathulenol were previously reported and could be investigated for preventing purposes and/or as adjuvant in the bovine mastitis treatment
Subject: Acremonium sp.
Agentes antibacterianos
Baccharis dracunculifolia L
Metabólitos
Essências e óleos essenciais
Aplysina fulva
Language: Multilíngua
Editor: [s.n.]
Citation: MARTINS, Thamires. Bovine mastitis: natural products as alternatives for treatment =Mastite bovina : produtos naturais como alternativas de tratamento . 2018. 1 recurso online ( 180 p.) Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos, Campinas, SP.
Date Issue: 2018
Appears in Collections:FEA - Tese e Dissertação

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