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Type: Artigo
Title: Isolation of spilanthol from acmella oleracea based on green chemistry and evaluation of its in vitro anti-inflammatory activity
Author: Blanco, Veronica Santana de Freitas
Michalak, Barbara
Zelioli, Ícaro Augusto Maccari
Oliveira, Adriana da Silva Santos de
Rodrigues, Marili Villa Nova
Ferreira, Antônio Gilberto
Garcia, Vera Lucia
Cabral, Fernando Antônio
Kiss, Anna Karolina
Rodrigues, Rodney Alexandre Ferreira
Abstract: Acmella oleracea (jambu) is a South American plant whose pharmacological properties include antimalarial, anesthetic, and anti-inflammatory activities. However, the compounds contributing to the anti-inflammatory effect have received little attention. In this study, the active compound spilanthol was isolated using a Green Chemistry approach and presented significant anti-inflammatory activity in a lipopolysaccharide-activated human neutrophil model. First, the aerial parts of jambu were extracted and fractionated using supercritical carbon dioxide. The spilanthol-enriched fractions obtained were then subjected to further purification by flash chromatography, yielding spilanthol with 97% purity. Use of enzyme-linked immunosorbent assay (ELISA) revealed a reduction in the release of Interleukin-8 and Tumor Necrosis Factor - alpha by leukocytes exposed to spilanthol. In conclusion, this new approach enabled spilanthol to be obtained at high purity, in a fairly rapid procedure, while the results of the in vitro anti-inflammatory activity study indicated that the compound could be a promising new therapeutic agent
Subject: Ensaio de imunoadsorção enzimática
Country: Países Baixos
Editor: Elsevier
Rights: Fechado
Identifier DOI: 10.1016/j.supflu.2018.07.028
Date Issue: 2018
Appears in Collections:FEA - Artigos e Outros Documentos
CPQBA - Artigos e Outros Documentos
FOP - Artigos e Outros Documentos

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