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Type: Artigo
Title: Bone and skin tissue engineering based on cytotoxicity assays of PLLA fibrous mats synthesized via centrifugal spinning
Author: Ciocca, Bárbara Etruri
Miranda, Nahieh Toscano
Cardoso, Guinea Brasil Camargo
Rodrigues, Ana Amélia
Pattaro, Ana Flávia
Munhoz, André Luiz Jardini
Maciel Filho, Rubens
Abstract: Poly (L-lactic acid) (PLLA) is a biocompatible polymer widely used in a range of applications and is already recognized in the medical field as a biomaterial. The main objective of the present work was the production of polymeric mats to be applied in tissue engineering. PLLA mats were produced through the centrifugal spinning process. Scanning electron microscopy (SEM) analyzed the fibers diameter, disposition, and morphology. The fibers presented a uniform and constant diameter (16.24 µm), being arranged randomly. Thermogravimetric analysis (TGA) technique verified variation of mass loss with temperature, in which the material degradation temperature started at 205 °C. Differential scanning calorimetry (DSC) determined phase transition temperatures. Fourier-transform infrared spectroscopy (FT-IR) evaluated PLLA fibrous membrane chemical groups. Cytotoxicity assays verified fibroblasts and osteoblasts viabilities by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide), AlamarBlue®, and Live/Dead® for 24, 48, and 72 h. This work results demonstrated that PLLA mats have great potential for tissue engineering applications in wound healing and bone repairing, being a promising biomaterial with high biocompatibility
Subject: PLLA
Country: Romênia
Editor: Bridge Center
Rights: Fechado
Date Issue: 2020
Appears in Collections:FEQ - Artigos e Outros Documentos

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