Please use this identifier to cite or link to this item:
Type: Artigo
Title: The effect of NaCl on the adsorption of human IgG onto CM-Asp–PEVA hollow fiber membrane-immobilized nickel and cobalt metal ions
Author: Pavan, Gisele Luiza
Lazzarotto Bresolin, Igor Tadeu
Borsoi-Ribeiro, Mariana
Vijayalakshmi, Mookambesvaran
Alves Bueno, Sonia Maria
Abstract: Over the past decade, immobilized metal-affinity adsorbents have attracted increasing interest for purification of natural and recombinant immunoglobulin G (IgG). In this work, nickel and cobalt metal ions complexed with CM-Asp (carboxymethylaspartate) immobilized on poly(ethylenevinyl alcohol) (PEVA) hollow fiber membranes were evaluated for purification of human IgG from serum. The buffer system and NaCl had important effects on human serum protein adsorption in both adsorbents. Efficient purification of IgG was accomplished in sodium phosphate buffer without NaCl at pH 7.0. Under this condition, the electrostatic interactions are important for adsorption. The Ni(II)-CM-Asp-PEVA had a protein adsorption capacity of 17.5 mg of IgG mL(-1) fiber when human serum diluted was loaded in crossflow filtration mode and the eluted IgG had a purity of 82.6 % (based on total protein and IgG, IgM, HSA, and Trf nephelometric analysis). Fitting the experimental IgG adsorption data to the Langmuir and Langmuir-Freundlich models showed that Ni(II)-CM-Asp and Co(II)-CM-Asp had Langmuirean and non-Langmuirean behavior, respectively, with positive cooperativity for IgG-Co(II)-CM-Asp binding, probably due to multipoint interactions (n = 2.12 +/- A 0.31). Thus, these membranes can be considered as alternative adsorbents for the purification or depletion of IgG from human serum
Subject: Imunoglobulina G
Country: Estados Unidos
Editor: Springer
Rights: Fechado
Identifier DOI: 10.1007/s10450-014-9612-1
Date Issue: 2014
Appears in Collections:FEQ - Artigos e Outros Documentos

Files in This Item:
File Description SizeFormat 
000339103300003.pdf808.83 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.