Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/349885
Type: Artigo
Title: Dual-oriented solid-phase molecular imprinting: toward selective artificial receptors for recognition of nucleotides in water
Author: Mourao, Cecilia A.
Bokeloh, Frank
Xu, Jingjing
Prost, Elise
Duma, Luminita
Merlier, Franck
Bueno, Sonia M. A.
Haupt, Karsten
Bui, Bernadette Tse Sum
Abstract: We describe the synthesis of water-soluble molecularly imprinted polymer nanoparticles (MIP-NPs) as a new artificial host receptor for the recognition of adenosine monophosphate (AMP), used herein, as a model nucleotide. MIP-NPs were prepared by solid-phase synthesis on glass beads (GB) using, for the first time, immobilized Fe(III)-chelate as an affinity ligand to orientate the AMP via its phosphate group. A polymerizable thymine monomer which can induce complementary base-pairing with the adenine moiety of the nucleotide was synthesized and incorporated in the polymerization mixture to constrain the AMP in a dual orientated configuration. The MIP-NPs were remarkably selective toward AMP as they did not bind other nucleotides, GMP, UMP, and CMP. This strategy of using the phosphate group of AMP as a hinge enables unhindered pairing of the nucleobase with its corresponding complementary base monomer and can be extended to the preparation of specific MIP receptors for other key nucleotides in aqueous conditions
Subject: Polimerização
Country: Estados Unidos
Editor: American Chemical Society
Rights: Fechado
Identifier DOI: 10.1021/acs.macromol.7b01782
Address: https://pubs.acs.org/doi/10.1021/acs.macromol.7b01782
Date Issue: 2017
Appears in Collections:FEQ - Artigos e Outros Documentos

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