Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/349783
Type: Artigo
Title: Screening of potential endoglucanases, hydrolysis conditions and different sugarcane straws pretreatments for cello-oligosaccharides production
Author: Barbosa, Fernando César
Martins, ManoelaMartins
Brenelli, Lívia BeatrizBrenelli
Ferrari, Felipe Augusto
Forte, Marcus Bruno Soares
Rabelo, Sarita Cândida
Franco, Telma Teixeira
Goldbeck, Rosana
Abstract: Cello-oligosaccharides (COS) are oligomers with 2 to 6 β-1,4-linked glucose units, with potential applications in the food/feed and bioenergy industrial sectors. In this study, the combination of five heterologous expressed endoglucanases varying the temperature and pH conditions were evaluated by design of experiments for COS production. Afterwards, the best combination was tested to produce COS from different pretreated sugarcane straws: ionic liquid, diluted acid, hydrothermal and steam-explosion. The results showed that steam explosion pretreated sugarcane straw treated with CtCel9R enzyme at 50 °C and pH 5.0 yielded 13.4 mg COS g biomass−1, 5–18-fold higher compared to the other pretreated straws. Under the conditions evaluated, the removal of hemicellulose and decrease in the cellulose crystallinity can benefits the enzymatic hydrolysis. This is the first study that combined the evaluation of different enzymes, conditions, and sugarcane straw pretreatments to optimize COS production in a single step without glucose formation
Subject: Endoglucanases
Country: Países Baixos
Editor: Elsevier
Rights: Fechado
Identifier DOI: 10.1016/j.biortech.2020.123918
Address: https://www.sciencedirect.com/science/article/pii/S0960852420311901
Date Issue: 2020
Appears in Collections:FEA - Artigos e Outros Documentos
FEQ - Artigos e Outros Documentos
NIPE - Artigos e Outros Documentos

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