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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.identifier.isbn978-88-95608-29-7pt_BR
dc.contributor.authorunicampSilva, Thiago Lopes da-
dc.contributor.authorunicampSilva Junior, Absolon Carvalho da-
dc.contributor.authorunicampVieira, Melissa Gurgel Adeodato-
dc.contributor.authorunicampSilva, Meuris Gurgel Carlos da-
dc.typeArtigopt_BR
dc.titleEvaluation of molecular weight distribution of sericin in solutions concentrated via precipitation by ethanol and precipitation by freezing/thawingpt_BR
dc.contributor.authorda Silva, Thiago L.-
dc.contributor.authorda Silva Junior, Absolon C.-
dc.contributor.authorRibani, Marcelo-
dc.contributor.authorVieira, Melissa G. A.-
dc.contributor.authorGimenes, Marcelino L.-
dc.contributor.authorda Silva, Meuris G. C.-
dc.subjectEtanolpt_BR
dc.subject.otherlanguageEthanolpt_BR
dc.description.abstractSericin is a globular water-soluble protein that constitutes 25 to 30 % of silk proteins weight in silkworm`s cocoons. This protein envelopes silk fibers gluing them and ensuring the cohesion of the cocoon. During raw silk production, sericin is separated from silk fibers and it is discarded in wastewater, leading to a high biological oxygen demand to be degraded. The sericin molecular weight ranges from 10 to 400 kDa and the structure and molecular weight depend on the method which is extracted and purified. Lower molecular weight sericin peptides are mainly used in cosmetic products, whereas higher molecular ones enable its use for many other applications, like, medical biomaterials, functional bio membranes, and others. This study evaluates the molecular weight distribution of sericin in solutions in which this protein was concentrated by precipitation by ethanol and precipitation by freezing/thawing. The solutions, which were subjected to precipitations, were filtered and the two solutions obtained (the concentrated one and the permeated one) had their molecular weight distribution analyzed. The sericin molecular weight distribution in the solutions was analyzed by size exclusion chromatography (SEC). In this analysis, the mobile phase is ultra-pure water (Milli-Q) and a standard curve was made with pullulan polysaccharide standards. The results showed that the degumming process in autoclave (1 kgf/cm2 – 40 min) was able to extract high molecular weight sericin and both precipitation methods could concentrate sericin in solution with no significant change in the molecular weight distribution, which ranges from lower than 20 kDa and up to 400 kDa valuespt_BR
dc.relation.ispartofChemical engineering transactionspt_BR
dc.publisher.cityMilanpt_BR
dc.publisher.countryItáliapt_BR
dc.publisherAssociazione Italiana di Ingegneria Chimicapt_BR
dc.date.issued2014-
dc.language.isoengpt_BR
dc.description.volume38pt_BR
dc.description.firstpage103pt_BR
dc.description.lastpage108pt_BR
dc.rightsFechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.eissn2283-9216pt_BR
dc.identifier.doi10.3303/CET1438018pt_BR
dc.identifier.urlhttps://www.cetjournal.it/index.php/cet/article/view/CET1438018pt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumbersem informaçãopt_BR
dc.date.available2020-09-16T13:03:22Z-
dc.date.accessioned2020-09-16T13:03:22Z-
dc.description.provenanceSubmitted by Sanches Olivia (olivias@unicamp.br) on 2020-09-16T13:03:22Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-01-11T20:02:04Z : No. of bitstreams: 1 000346539600019.pdf: 895886 bytes, checksum: fb60be6720931167555dc3f53b0a5abc (MD5)en
dc.description.provenanceMade available in DSpace on 2020-09-16T13:03:22Z (GMT). No. of bitstreams: 0 Previous issue date: 2014en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/349384-
dc.description.conferencenome4th International Conference on Industrial Biotechnology (IBIC2014)pt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentDepartamento de Desenvolvimento de Processos e Produtospt_BR
dc.contributor.departmentDepartamento de Desenvolvimento de Processos e Produtospt_BR
dc.contributor.unidadeFaculdade de Engenharia Químicapt_BR
dc.identifier.source000346539600019pt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcid0000-0002-2171-2061pt_BR
dc.creator.orcid0000-0002-3487-799Xpt_BR
dc.creator.orcid0000-0003-1000-1392pt_BR
dc.type.formArtigo de pesquisapt_BR
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