Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/349306
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampFreitas, José Américo Nabuco Leva Ferreira de-
dc.contributor.authorunicampLeomil, Fernanda dos Santos Costa-
dc.contributor.authorunicampZoccoler, Marcelo-
dc.contributor.authorunicampAntoneli, Priscila Correia-
dc.contributor.authorunicampOliveira, Pedro Xavier de-
dc.typeArtigopt_BR
dc.titleCardiomyocyte lethality by multidirectional stimulipt_BR
dc.contributor.authorFreitas, José Américo Nabuco Leva Ferreira de-
dc.contributor.authorLeomil, Fernanda dos Santos Costa-
dc.contributor.authorZoccoler, Marcelo-
dc.contributor.authorAntoneli, Priscila Correia-
dc.contributor.authorOliveira, Pedro Xavier de-
dc.subjectDesfibriladorespt_BR
dc.subject.otherlanguageDefibrillatorspt_BR
dc.description.abstractMultidirectional defibrillation protocols have shown better efficiency than monodirectional; still, no testing was performed to assess cell lethality. We investigated lethality of multidirectional defibrillator-like shocks on isolated cardiomyocytes. Cells were isolated from adult male Wistar rats and plated into a perfusion chamber. Electrical field stimulation threshold (ET) was obtained, and cells were paced with suprathreshold bipolar electrical field (E) pulses. Either one monodirectional high-intensity electrical field (HEF) pulse aligned at 0° (group Mono0) or 60° (group Mono60) to cell major axis or a multidirectional sequence of three HEF pulses aligned at 0°, 60°, and 120° each was applied. If cell recovered from shock, pacing was resumed, and a higher amplitude HEF, proportional to ET, was applied. The sequence was repeated until cell death. Lethality curves were built by means of survival analysis from sub-lethal and lethal E. Non-linear fit was performed, and E values corresponding to 50% probability of lethality (E50) were compared. Multidirectional groups presented lethality curves similar to Mono0. Mono60 displayed the highest E50. The novel data endorse the idea of multidirectional stimuli being safer because their effects on lethality of individual cells were equal to a single monodirectional stimulus, while their defibrillatory threshold is lowerpt_BR
dc.relation.ispartofMedical & biological engineering & computingpt_BR
dc.relation.ispartofabbreviationMed. biol. eng. comput.pt_BR
dc.publisher.cityHeidelbergpt_BR
dc.publisher.countryAlemanhapt_BR
dc.publisherSpringerpt_BR
dc.date.issued2018-
dc.date.monthofcirculationDec.pt_BR
dc.language.isoengpt_BR
dc.description.volume56pt_BR
dc.description.firstpage2177pt_BR
dc.description.lastpage2184pt_BR
dc.rightsFechadopt_BR
dc.sourcePUBMEDpt_BR
dc.identifier.issn0140-0118pt_BR
dc.identifier.eissn1741-0444pt_BR
dc.identifier.doi10.1007/s11517-018-1848-6pt_BR
dc.identifier.urlhttps://link.springer.com/article/10.1007%2Fs11517-018-1848-6pt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsorshipCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumbernão tempt_BR
dc.description.sponsordocumentnumbernão tempt_BR
dc.description.sponsordocumentnumber2014/18.798-1pt_BR
dc.date.available2020-09-15T16:03:39Z-
dc.date.accessioned2020-09-15T16:03:39Z-
dc.description.provenanceSubmitted by Mariana Aparecida Azevedo (mary1@unicamp.br) on 2020-09-15T16:03:39Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-01-08T19:03:12Z : No. of bitstreams: 1 29845489.pdf: 1252228 bytes, checksum: 68f00dbaebf6c733c2e3552a7df4aae6 (MD5)en
dc.description.provenanceMade available in DSpace on 2020-09-15T16:03:39Z (GMT). No. of bitstreams: 0 Previous issue date: 2018en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/349306-
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentDepartamento de Engenharia Biomédicapt_BR
dc.contributor.unidadeFaculdade de Engenharia Elétrica e de Computaçãopt_BR
dc.contributor.unidadeFaculdade de Engenharia Elétrica e de Computaçãopt_BR
dc.contributor.unidadeFaculdade de Engenharia Elétrica e de Computaçãopt_BR
dc.contributor.unidadeFaculdade de Engenharia Elétrica e de Computaçãopt_BR
dc.contributor.unidadeFaculdade de Engenharia Elétrica e de Computaçãopt_BR
dc.subject.keywordIsolated ventricular myocytespt_BR
dc.subject.keywordElectrical field stimulationpt_BR
dc.identifier.source29845489pt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcid0000-0002-6165-4679pt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcid0000-0002-1944-7481pt_BR
dc.type.formArtigo originalpt_BR
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