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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampDavel, Ana Paula-
dc.typeArtigopt_BR
dc.titleL-NAME treatment enhances exercise-induced content of myocardial heat shock protein 72 (Hsp72) in ratspt_BR
dc.contributor.authorLunz, Wellington-
dc.contributor.authorCapettini, Luciano S. A.-
dc.contributor.authorDavel, Ana P. C.-
dc.contributor.authorMunhoz, Carolina D.-
dc.contributor.authorda Silva, Josiane F.-
dc.contributor.authorRossoni, Luciana V.-
dc.contributor.authorLemos, Virginia S.-
dc.contributor.authorBaldo, Marcelo P.-
dc.contributor.authorCarneiro-Junior, Miguel A.-
dc.contributor.authorNatali, Antonio J.-
dc.contributor.authorde Lacerda, Luiz H. S.-
dc.contributor.authorMill, Jose G.-
dc.subjectÓxido nítricopt_BR
dc.subject.otherlanguageNitric oxidept_BR
dc.description.abstractBackground/Aim: Nitric oxide (NO) modulates the expression of the chaperone Hsp72 in the heart, and exercise stimulates both NO production and myocardial Hsp72 expression. The main purpose of the study was to investigate whether NO interferes with an exercise-induced myocardial Hsp72 expression. Methods: Male Wistar rats (70-100 days) were divided into control (C, n= 12), L-NAME-treated (L, n= 12), exercise (E, n= 13) and exercise plus L-NAME-treated (EL, n= 20) groups. L-NAME was given in drinking water (700 mg. L-1) and the exercise was performed on a treadmill (15-25 m.min(-1), 40-60 min. day(-1)) for seven days. Left ventricle (LV) protein Hsp content, NOS and phosphorylated-NOS (p-NOS) isoforms were measured using Western blotting. The activity of NOS was assayed in LV homogenates by the conversion of [H-3] L-arginine to [H-3] L-citrulline. Results: Hsp72 content was increased significantly (223%; p < 0.05) in the E group compared to the C group, but exercise alone did not alter the NOS content, p-NOS isoforms or NOS activity. Contrary to our expectation, L-NAME enhanced (p < 0.05) the exercise-induced Hsp72 content (EL vs. C, L and E groups = 1019%, 548% and 457%, respectively). Although the EL group had increased stimulatory p-eNOS(Ser1177) (over 200%) and decreased inhibitory p-nNOS(Ser852) (similar to 50%) compared to both the E and L groups (p < 0.05), NOS activity was similar in all groups. Conclusions: Our results suggest that exercise-induced cardiac Hsp72 expression does not depend on NO. Conversely, the in vivo L-NAME treatment enhances exercise-induced Hsp72 production. This effect may be due to an increase in cardiac stress. Copyright (C) 2011 S. Karger AG, Baselpt_BR
dc.relation.ispartofCellular physiology and biochemistrypt_BR
dc.relation.ispartofabbreviationCell physiol biochempt_BR
dc.publisher.cityDüsseldorfpt_BR
dc.publisher.countryAlemanhapt_BR
dc.publisherCellular Physiology and Biochemistrypt_BR
dc.date.issued2011-
dc.language.isoengpt_BR
dc.description.volume27pt_BR
dc.description.issuenumber5pt_BR
dc.description.firstpage479pt_BR
dc.description.lastpage486pt_BR
dc.rightsAbertopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn1015-8987pt_BR
dc.identifier.eissn1421-9778pt_BR
dc.identifier.doi10.1159/000329969pt_BR
dc.identifier.urlhttps://www.karger.com/Article/Abstract/329969pt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA E INOVAÇÃO DO ESPÍRITO SANTO - FAPESpt_BR
dc.description.sponsordocumentnumbersem informaçãopt_BR
dc.date.available2020-09-10T11:07:34Z-
dc.date.accessioned2020-09-10T11:07:34Z-
dc.description.provenanceSubmitted by Sanches Olivia (olivias@unicamp.br) on 2020-09-10T11:07:34Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-01-04T15:13:11Z : No. of bitstreams: 1 000291754000007.pdf: 709318 bytes, checksum: 1082bb3f41d26352d17c180bd25970ac (MD5)en
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dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/349012-
dc.contributor.departmentDepartamento de Biologia Estrutural e Funcionalpt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.subject.keywordHsp72pt_BR
dc.subject.keywordExercisept_BR
dc.subject.keywordNitric oxide synthasept_BR
dc.identifier.source000291754000007pt_BR
dc.creator.orcid0000-0002-9862-4262pt_BR
dc.type.formArtigo originalpt_BR
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