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Type: Artigo
Title: LC-MS/MS quantitation of plasma progesterone in cattle
Author: Fernandes, R.M.T.
Gomes, G.C.
Porcari, A.M.
Pimentel, J.R.V.
Porciúncula, P.M.
Martins-Júnior, H.A.
Miguez, P.H.P.
da Costa, J.L.
Amaral, P.H.
Perecin, F.
Meurer, E.C.
Furtado, P.V.
Simas, R.C.
Eberlin, M.N.
Ferreira, C.R.
Madureira, E.H.
Abstract: Quantitation of progesterone (P4) in biological fluids is often performed by radioimmunoassay (RIA), whereas liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) has been used much less often. Due to its autoconfirmatory nature, LC-MS/MS greatly minimizes false positives and interference. Herein we report and compare with RIA an optimized LC-MS/MS method for rapid, efficient, and cost-effective quantitation of P4 in plasma of cattle with no sample derivatization. The quantitation of plasma P4 released from three nonbiodegradable, commercial, intravaginal P4-releasing devices (IPRD) over 192 h in six ovariectomized cows was compared in a pairwise study as a test case. Both techniques showed similar P4 kinetics (P > 0.05) whereas results of P4 quantitation by RIA were consistently higher compared with LC-MS/MS (P < 0.05) due to interference and matrix effects. The LC-MS/MS method was validated according to the recommended analytical standards and displayed P4 limits of detection (LOD) and quantitation (LOQ) of 0.08 and a 0.25 ng/mL, respectively. The high selective LC-MS/MS method proposed herein for P4 quantitation eliminates the risks associated with radioactive handling; it also requires no sample derivatization, which is a common requirement for LC-MS/MS quantitation of steroid hormones. Its application to multisteroid assays is also viable, and it is envisaged that it may provide a gold standard technique for hormone quantitation in animal reproductive science studies.
Subject: LC-MS/MS
Country: Estados Unidos
Editor: Elsevier
Rights: Fechado
Identifier DOI: 10.1016/j.theriogenology.2011.05.033
Date Issue: 2011
Appears in Collections:IQ - Artigos e Outros Documentos

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