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Type: Artigo
Title: Quantification of chlorpheniramine maleate enantiomers by ultraviolet spectroscopy and chemometric methods
Author: Valderrama, P.
Romero, A.L.
Imamura, P.M.
Magalhães, I.R.S.
Bonato, P.S.
Poppi, R.J
Abstract: Chlorpheniramine maleate (CLOR) enantiomers were quantified by ultraviolet spectroscopy and partial least squares regression. The CLOR enantiomers were prepared as inclusion complexes with β-cyclodextrin and 1-butanol with mole fractions in the range from 50 to 100%. For the multivariate calibration the outliers were detected and excluded and variable selection was performed by interval partial least squares and a genetic algorithm. Figures of merit showed results for accuracy of 3.63 and 2.83% (S)-CLOR for root mean square errors of calibration and prediction, respectively. The ellipse confidence region included the point for the intercept and the slope of 1 and 0, respectively. Precision and analytical sensitivity were 0.57 and 0.50% (S)-CLOR, respectively. The sensitivity, selectivity, adjustment, and signal-to-noise ratio were also determined. The model was validated by a paired t test with the results obtained by high-performance liquid chromatography proposed by the European pharmacopoeia and circular dichroism spectroscopy. The results showed there was no significant difference between the methods at the 95% confidence level, indicating that the proposed method can be used as an alternative to standard procedures for chiral analysis.
Subject: Enantiômeros
Calibração multivariada
Espectroscopia ultravioleta
Country: Alemanha
Editor: Springer
Rights: Fechado
Identifier DOI: 10.1007/s00216-010-3472-7
Date Issue: 2010
Appears in Collections:IQ - Artigos e Outros Documentos

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