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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampUeta, Marlene Tiduko-
dc.typeArtigopt_BR
dc.titleThe ubiquitin–proteasome system in Strongyloididae. Biochemical evidence for developmentally regulated proteolysis in Strongyloides venezuelensispt_BR
dc.contributor.authorde Paula, Fabiana M.-
dc.contributor.authorCastro-Borges, William-
dc.contributor.authorPereira Junior, Olavo S.-
dc.contributor.authorGomes, Matheus de Souza-
dc.contributor.authorUeta, Marlene T.-
dc.contributor.authorRodrigues, Vanderlei-
dc.subjectVia ubiquitina-proteossomopt_BR
dc.subject.otherlanguageUbiquitin-proteasomal pathwaypt_BR
dc.description.abstractNematode parasites from the genus Strongyloides spp. are important pathogens of the intestinal mucosa of animals and humans. Their complex life cycles involve alternating developmental adaptations between larvae stages and the adult parthenogenetic female. Here, we report, primarily through homology-based searching, the existence of the major components of the ubiquitin-proteasome system in this genus, using the available EST data from S. ratti, S. stercoralis, and Parastrongyloides trichosuri. In this study, S. venezuelensis was used as our model organism for detection of proteasome activity and ubiquitinated substrates in cytosolic preparations from the L3 larvae and the adult female. Marked differences in proteasome capabilities were found when these two stages were compared. A preference for degradation of chymotryptic synthetic peptides was found in both stages with the adult exhibiting a higher rate of hydrolysis compared to the larvae. Due to the high evolutionary conservation of proteasome alpha subunits, an anti-human proteasome antibody was able to recognize proteasome subunits in these preparations by Western blotting, supporting the proposal that the activity of the ubiqutin-proteasome system is developmentally regulated in this nematodept_BR
dc.relation.ispartofParasitology researchpt_BR
dc.relation.ispartofabbreviationParasitol. res.pt_BR
dc.publisher.cityBerlinpt_BR
dc.publisher.countryAlemanhapt_BR
dc.publisherSpringerpt_BR
dc.date.issued2009-
dc.date.monthofcirculationAug.pt_BR
dc.language.isoengpt_BR
dc.description.volume105pt_BR
dc.description.issuenumber2pt_BR
dc.description.firstpage567pt_BR
dc.description.lastpage576pt_BR
dc.rightsFechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn0932-0113pt_BR
dc.identifier.eissn1432-1955pt_BR
dc.identifier.doi10.1007/s00436-009-1430-0pt_BR
dc.identifier.urlhttps://link.springer.com/article/10.1007/s00436-009-1430-0pt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsordocumentnumbersem informaçãopt_BR
dc.date.available2020-09-09T20:31:21Z-
dc.date.accessioned2020-09-09T20:31:21Z-
dc.description.provenanceSubmitted by Sanches Olivia (olivias@unicamp.br) on 2020-09-09T20:31:21Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-01-04T15:13:08Z : No. of bitstreams: 1 000267297300035.pdf: 320497 bytes, checksum: 1a6820c18e0ec5f528a81e2e88574c9a (MD5)en
dc.description.provenanceMade available in DSpace on 2020-09-09T20:31:21Z (GMT). No. of bitstreams: 0 Previous issue date: 2009en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/348987-
dc.contributor.departmentDepartamento de Biologia Animalpt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.subject.keywordParasitic nematodept_BR
dc.subject.keywordProteasome activitypt_BR
dc.subject.keywordProteasome pathwaypt_BR
dc.subject.keywordAntibody binding sitept_BR
dc.subject.keywordParthenogenetic femalept_BR
dc.identifier.source000267297300035pt_BR
dc.creator.orcid0000-0002-6604-4351pt_BR
dc.type.formArtigo originalpt_BR
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