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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampGozzo, Fábio Cesar-
dc.typeArtigopt_BR
dc.titleInhibition of thimet oligopeptidase by siRNA alters specific intracellular peptides and potentiates isoproterenol signal transductionpt_BR
dc.contributor.authorRusso, Lilian C.-
dc.contributor.authorCastro, Leandro M.-
dc.contributor.authorGozzo, Fabio C.-
dc.contributor.authorFerro, Emer S.-
dc.subjectRNA interferente pequenopt_BR
dc.subject.otherlanguageRNA, Small Interferingpt_BR
dc.description.abstractMammalian cells have a large number of intracellular peptides that are generated by extralysosomal proteases. In this study, the enzymatic activity of thimet oligopeptidase (EP24.15) was inhibited in human embryonic kidney (HEK) 293 cells using a specific siRNA sequence. The semi‐quantitative intracellular peptidome analyses of siRNA‐transfected HEK293 cells shows that the levels of specific intracellular peptides are either increased or decreased upon EP24.15 inhibition. Decreased expression of EP24.15 was sufficient to potentiate luciferase gene reporter activation by isoproterenol (1–10 μM). The protein kinase A inhibitor KT5720 (1 μM) reduced the positive effect of the EP24.15 siRNA on isoproterenol signaling. Thus, EP24.15 inhibition by siRNA modulates the levels of specific intracellular peptides and isoproterenol signal transductionpt_BR
dc.relation.ispartofFEBS letterspt_BR
dc.relation.ispartofabbreviationFEBS lettpt_BR
dc.publisher.cityOxfordpt_BR
dc.publisher.countryReino Unidopt_BR
dc.publisherJohn Wiley & Sonspt_BR
dc.date.issued2012-
dc.date.monthofcirculationSept.pt_BR
dc.language.isoengpt_BR
dc.description.volume586pt_BR
dc.description.issuenumber19pt_BR
dc.description.firstpage3287pt_BR
dc.description.lastpage3292pt_BR
dc.rightsFechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn0014-5793pt_BR
dc.identifier.eissn1873-3468pt_BR
dc.identifier.doi10.1016/j.febslet.2012.07.002pt_BR
dc.identifier.urlhttps://febs.onlinelibrary.wiley.com/doi/full/10.1016/j.febslet.2012.07.002pt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumber559698/2009-7pt_BR
dc.description.sponsordocumentnumberSem informaçãopt_BR
dc.date.available2020-09-09T16:31:18Z-
dc.date.accessioned2020-09-09T16:31:18Z-
dc.description.provenanceSubmitted by Thais de Brito Barroso (tbrito@unicamp.br) on 2020-09-09T16:31:18Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-01-04T15:13:05Z : No. of bitstreams: 1 000309314200044.pdf: 700726 bytes, checksum: c432a538b29569d2e0141f7a149a2d42 (MD5)en
dc.description.provenanceMade available in DSpace on 2020-09-09T16:31:18Z (GMT). No. of bitstreams: 0 Previous issue date: 2012en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/348959-
dc.contributor.departmentDepartamento de Química Orgânicapt_BR
dc.contributor.unidadeInstituto de Químicapt_BR
dc.subject.keywordOligopeptidasept_BR
dc.subject.keywordG-protein coupled receptorpt_BR
dc.subject.keywordsiRNApt_BR
dc.subject.keywordIntracellular peptidept_BR
dc.subject.keywordUbiquitinproteasome systempt_BR
dc.identifier.source000309314200044pt_BR
dc.creator.orcid0000-0002-5270-4427pt_BR
dc.type.formComunicaçãopt_BR
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