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Type: Artigo
Title: Nanoassisted laser desorption-ionization-ms imaging of tumors
Author: Tata, Alessandra
Fernandes, Anna Maria A. P.
Santos, Vanessa G.
Alberici, Rosana M.
Araldi, Dioneia
Parada, Carlos A.
Braguini, Wellington
Veronez, Luciana
Bisson, Gabriela Silva
Reis, Felippe H. Z.
Alberici, Luciane C.
Eberlin, Marcos N.
Abstract: The ability of nanoassisted laser desorption-ionization mass spectrometry (NALDI-MS) imaging to provide selective chemical monitoring with proper spatial distribution of lipid profiles from tumor tissues after plate imprinting has been tested. NALDI-MS imaging identified and mapped several potential lipid biomarkers in a murine model of melanoma tumor (inoculation of B16/F10 cells). It also confirmed that the in vivo treatment of tumor bearing mice with synthetic supplement containing phosphoethanolamine (PHO-S) promoted an accentuated decrease in relative abundance of the tumor biomarkers. NALDI-MS imaging is a matrix-free LDI protocol based on the selective imprinting of lipids in the NALDI plate followed by the removal of the tissue. It therefore provides good quality and selective chemical images with preservation of spatial distribution and less interference from tissue material. The test case described herein illustrates the potential of chemically selective NALDI-MS imaging for biomarker discovery
Subject: Ionização
Country: Estados Unidos
Editor: American Chemical Society
Rights: Fechado
Identifier DOI: 10.1021/ac301202q
Date Issue: 2012
Appears in Collections:IQ - Artigos e Outros Documentos
IB - Artigos e Outros Documentos

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