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Type: Artigo
Title: Rat forming incisor requires a rigorous ECM remodeling modulated by MMP/RECK balance
Author: Paiva, Katiucia Batista Silva
Zambuzzi, Willian Fernando
Accorsi-Mendonça, Thais
Taga, Rumio
Nunes, Fabio Daumas
Sogayar, Mari Cleide
Granjeiro, José Mauro
Abstract: Reversion-inducing-cysteine-rich protein with Kazal motifs (RECK) is a single membrane-anchored MMP-regulator and regulates matrix metalloproteinases (MMP) 2, 9 and 14. In turn, MMPs are endopeptidases that play a pivotal role in remodeling ECM. In this work, we decided to evaluate expression pattern of RECK in growing rat incisor during, specifically focusing out amelogenesis process. Based on different kinds of ameloblasts, our results showed that RECK expression was conducted by secretory and post-secretory ameloblasts. At the secretory phase, RECK was localized in the infra-nuclear region of the ameloblast, outer epithelium, near blood vessels, and in the stellate reticulum. From the transition to the maturation phases, RECK was strongly expressed by non-epithelial immuno-competent cells (macrophages and/or dendritic-like cells) in the papillary layer. From the transition to the maturation stage, RECK expression was increased. RECK mRNA was amplified by RT-PCR from whole enamel organ. Here, we verified the presence of RECK mRNA during all stages of amelogenesis. These events were governed by ameloblasts and by non-epithelial cells residents in the enamel organ. Concluding, we found differential expression of MMPs-2, -9 and RECK in the different phases of amelogenesis, suggesting that the tissue remodeling is rigorously controlled during dental mineralization
Subject: Metaloproteinases da matriz
Country: Países Baixos
Editor: Springer
Rights: Fechado
Identifier DOI: 10.1007/s10735-009-9231-4
Date Issue: 2009
Appears in Collections:IB - Artigos e Outros Documentos
FOP - Artigos e Outros Documentos

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