Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/348909
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampZambuzzi, Willian Fernando-
dc.typeArtigopt_BR
dc.titleMMP-9 and CD68+ cells are required for tissue remodeling in response to natural hydroxyapatitept_BR
dc.contributor.authorZambuzzi, Willian F.-
dc.contributor.authorPaiva, Katiúcia B. S.-
dc.contributor.authorMenezes, Renato-
dc.contributor.authorOliveira, Rodrigo C.-
dc.contributor.authorTaga, Rumio-
dc.contributor.authorGranjeiro, José M.-
dc.subjectBiomateriaispt_BR
dc.subject.otherlanguageBiomaterialspt_BR
dc.description.abstractLarge bone defects represent major clinical problems in the practice of reconstructive orthopedic and craniofacial surgery. The aim of this study was to examine, through immunohistochemistry approach, the involvement of MMP-9 and CD68+ cells during tissue remodeling in response to natural hydroxyapatite (HA) implanted in rat subcutaneous tissue. Before experimentation, forty animals were randomly distributed into two experimental groups: Group-I (Gen-Ox™ micro-granules) and Group-II (Gen-Ox™ macro-granules). Afterwards, the biopsies were collected after 10, 20, 30, and 60 days post-implantation. Our results showed that at 10 days, a low-renewal foreign body type granuloma formation was observed in most of the cases. Macrophage- and fibroblast-like cells were the predominant type of cells positively stained for MMP-9 in both groups. Once macrophage-like cells seemed to be the major source of MMP9, antibody against pan-CD68 epitope was used to correlate these findings. In agreement, MMP-9 and CD68+ cells were distributed at the periphery and the central region of the granuloma in all experimental periods, however no staining was observed in cell contacting to material. Besides macrophages, the lysosomal glycoprotein epitope recognized by CD68 antibodies can be expressed by mast cell granules and sometimes by fibroblasts. Taken together, our results suggest that xenogenic HA promotes extracellular matrix remodeling through induction of MMP-9 activity and presence of CD68+ cellspt_BR
dc.relation.ispartofJournal of molecular histologypt_BR
dc.relation.ispartofabbreviationJ. mol. histol.pt_BR
dc.publisher.cityDordrechtpt_BR
dc.publisher.countryPaíses Baixospt_BR
dc.publisherSpringerpt_BR
dc.date.issued2009-
dc.date.monthofcirculationAug.pt_BR
dc.language.isoengpt_BR
dc.description.volume40pt_BR
dc.description.firstpage301pt_BR
dc.description.lastpage309pt_BR
dc.rightsFechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn1567-2379pt_BR
dc.identifier.eissn1567-2387pt_BR
dc.identifier.doi10.1007/s10735-009-9241-2pt_BR
dc.identifier.urlhttps://link.springer.com/article/10.1007%2Fs10735-009-9241-2pt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumber08/53003-9pt_BR
dc.date.available2020-09-09T12:33:26Z-
dc.date.accessioned2020-09-09T12:33:26Z-
dc.description.provenanceSubmitted by Mariana Aparecida Azevedo (mary1@unicamp.br) on 2020-09-09T12:33:26Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-01-04T15:12:58Z : No. of bitstreams: 1 000272174800007.pdf: 755719 bytes, checksum: 5413b3ce40a63612e39d4a2a3e1543b7 (MD5)en
dc.description.provenanceMade available in DSpace on 2020-09-09T12:33:26Z (GMT). No. of bitstreams: 0 Previous issue date: 2009en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/348909-
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.subject.keywordXenograftspt_BR
dc.subject.keywordHydroxyapatitept_BR
dc.subject.keywordMMP-9pt_BR
dc.subject.keywordCD68 antigenpt_BR
dc.identifier.source000272174800007pt_BR
dc.creator.orcid0000-0002-4149-5965pt_BR
dc.type.formArtigo originalpt_BR
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