Please use this identifier to cite or link to this item:
Type: Artigo
Title: A role for galectin‐3 in renal tissue damage triggered by ischemia and reperfusion injury
Author: Bertocchi, Ana Paula Fernandes
Campanhole, Gabriela
Wang, Pamella Huey Mei
Gonçalves, Giselle Martins
Damião, Márcio José
Cenedeze, Marcos Antônio
Beraldo, Felipe Caetano
Teixeira, Vicente De Paula Antunes
Reis, Marlene Antônia Dos
Mazzali, Marilda
Pacheco‐Silva, Alvaro
Câmara, Niels Olsen Saraiva
Abstract: Ischemic‐reperfusion injury (IRI) triggers an inflammatory response involving neutrophils/macrophages, lymphocytes and endothelial cells. Galectin‐3 is a multi‐functional lectin with a broad range of action such as promotion of neutrophil adhesion, induction of oxidative stress, mastocyte migration and degranulation, and production of pro‐inflammatory cytokines. The aim of this study was evaluate the role of galectin‐3 in the inflammation triggered by IRI. Galectin‐3 knockout (KO) and wild type (wt) mice were subjected to 45 min of renal pedicle occlusion. Blood and kidney samples were collected at 6, 24, 48 and 120 h. Blood urea was analyzed enzymatically, while MCP‐1, IL‐6 and IL‐1β were studied by real‐time PCR. Reactive oxygen species (ROS) was investigated by flow cytometry. Morphometric analyses were performed at 6, 24, 48 and 120 h after reperfusion. Urea peaked at 24 h, being significantly lower in knockout animals (wt = 264.4 ± 85.21 mg/dl vs. gal‐3 KO = 123.74 ± 29.64 mg/dl, P = 0.001). Galectin‐3 knockout animals presented less acute tubular necrosis and a more prominent tubular regeneration when compared with controls concurrently with lower expression of MCP‐1, IL‐6, IL‐1β, less macrophage infiltration and lower ROS production at early time points. Galectin‐3 seems to play a role in renal IRI involving the secretion of macrophage‐related chemokine, pro‐inflammatory cytokines and ROS production
Subject: Reperfusão
Country: Reino Unido
Editor: Wiley
Rights: Fechado
Identifier DOI: 10.1111/j.1432-2277.2008.00705.x
Date Issue: 2008
Appears in Collections:FCM - Artigos e Outros Documentos

Files in This Item:
File Description SizeFormat 
000259087800012.pdf450.11 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.