Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/348854
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampSouza, Gustavo Henrique Martins Ferreira de-
dc.contributor.authorunicampEberlin, Marcos Nogueira-
dc.contributor.authorunicampHyslop, Stephen-
dc.typeArtigopt_BR
dc.titleCharacterization of the mechanisms underlying the inflammatory response to Polistes lanio lanio (paper wasp) venom in mouse dorsal skinpt_BR
dc.contributor.authorYshii, Lídia M.-
dc.contributor.authorSouza, Gustavo H.M.F.-
dc.contributor.authorCamargo, Enilton A.-
dc.contributor.authorEberlin, Marcos N.-
dc.contributor.authorRibela, Maria Teresa C.P.-
dc.contributor.authorMuscará, Marcelo N.-
dc.contributor.authorHyslop, Stephen-
dc.contributor.authorCosta, Soraia K.P.-
dc.subjectHistaminapt_BR
dc.subject.otherlanguageHistaminept_BR
dc.description.abstractStings by Polistes wasps can cause life-threatening allergic reactions, pain and inflammation. We examined the changes in microvascular permeability and neutrophil influx caused by the venom of Polistes lanio a paper wasp found in southeastern Brazil. The intradermal injection of wasp venom caused long-lasting paw oedema and dose-dependently increased microvascular permeability in mouse dorsal skin. SR140333, an NK1 receptor antagonist, markedly inhibited the response, but the NK2 receptor antagonist SR48968 was ineffective. The oedema was reduced in capsaicin-treated rats, indicating a direct activation of sensory fibres. Dialysis of the venom partially reduced the oedema and the remaining response was further inhibited by SR140333. Mass spectrometric analysis of the venom revealed two peptides (QPPTPPEHRFPGLM and ASEPTALGLPRIFPGLM) with sequence similarities to the C-terminal region of tachykinin-like peptides found in Phoneutria nigriventer spider venom and vertebrates. Wasp venom failed to release histamine from mast cells in vitro and spectrofluorometric assay of the venom revealed a negligible content of histamine in the usual dose of P. l. lanio venom (1 nmol of histamine/7 μg of venom) that was removed by dialysis. The histamine H1 receptor antagonist pyrilamine, but not bradykinin B1 or B2 receptor antagonists, inhibited venom-induced oedema. In conclusion, P. l. lanio venom induces potent oedema and increases vascular permeability in mice, primarily through activation of tachykinin NK1 receptors by substance P released from sensory C fibres, which in turn releases histamine from dermal mast cells. This is the first description of a neurovascular mechanism for P. l. lanio venom-mediated inflammation. The extent to which the two tachykinin-like peptides identified here contribute to this neurogenic inflammatory response remains to be elucidatedpt_BR
dc.relation.ispartofToxicon: an interdisciplinary journal on the toxins derived from animals, plants and microorganismspt_BR
dc.relation.ispartofabbreviationToxiconpt_BR
dc.publisher.cityOxfordpt_BR
dc.publisher.countryReino Unidopt_BR
dc.publisherElsevierpt_BR
dc.date.issued2009-
dc.date.monthofcirculationJan.pt_BR
dc.language.isoengpt_BR
dc.description.volume53pt_BR
dc.description.issuenumber1pt_BR
dc.description.firstpage42pt_BR
dc.description.lastpage52pt_BR
dc.rightsFechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn0041-0101pt_BR
dc.identifier.eissn1879-3150pt_BR
dc.identifier.doi10.1016/j.toxicon.2008.10.006pt_BR
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S0041010108005448pt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsorshipCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESpt_BR
dc.description.sponsorshipFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPpt_BR
dc.description.sponsordocumentnumberNão tempt_BR
dc.description.sponsordocumentnumberNão tempt_BR
dc.description.sponsordocumentnumberNão tempt_BR
dc.date.available2020-09-08T16:16:30Z-
dc.date.accessioned2020-09-08T16:16:30Z-
dc.description.provenanceSubmitted by Susilene Barbosa da Silva (susilene@unicamp.br) on 2020-09-08T16:16:30Z No. of bitstreams: 0. Added 1 bitstream(s) on 2021-01-04T15:12:52Z : No. of bitstreams: 1 000262774100006.pdf: 499846 bytes, checksum: 7e1447a4d11c32c775776c8b43336978 (MD5)en
dc.description.provenanceMade available in DSpace on 2020-09-08T16:16:30Z (GMT). No. of bitstreams: 0 Previous issue date: 2009en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/348854-
dc.contributor.departmentSem informaçãopt_BR
dc.contributor.departmentDepartamento de Química Orgânicapt_BR
dc.contributor.departmentDepartamento de Farmacologiapt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.contributor.unidadeInstituto de Químicapt_BR
dc.contributor.unidadeFaculdade de Ciências Médicaspt_BR
dc.description.abstractalternativeHistaminePeptidePlasma extravasationMast cellWasp venompt_BR
dc.subject.keywordNK1 receptorspt_BR
dc.subject.keywordPeptidept_BR
dc.subject.keywordPlasma extravasationpt_BR
dc.subject.keywordMast cellpt_BR
dc.identifier.source000262774100006pt_BR
dc.creator.orcidSem informaçãopt_BR
dc.creator.orcid0000-0003-4868-0618pt_BR
dc.creator.orcid0000-0002-9293-3638pt_BR
dc.type.formArtigopt_BR
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